黑、红花生衣中原花色素的分析.pdfVIP

190 2014, Vol.35, No.04 食品科学 ※分析检测 黑、红花生衣中原花色素的分析 杜 蕾，李新华* （沈阳农业大学食品学院，辽宁沈阳 110866 ） 摘 要：对黑、红花生衣中的原花色素成分进行考察。通过乙醇溶液提取、有机溶剂萃取分离以及大孔树脂纯化的方 法对花生衣色素成分进行分离制备，采用高效液相色谱法对色素中原花色素的含量进行测定，并应用高效液相色谱- 质谱联用技术对原花色素成分的组成进行鉴定。黑、红花生衣色素中原花色素的含量分别为29.19%和43.04% 。依据质 谱信息初步鉴定出红花生衣中含有4 种原花色素二聚体和4 种原花色素三聚体，黑花生衣中含有3 种原花色素二聚体 和4 种原花色素三聚体。该法测定原花色素含量简便、快捷，可以较好的鉴定花生衣中原花色素的主要成分。 关键词：原花色素；高效液相色谱法；液相色谱-质谱联用；黑花生衣；红花生衣 Analysis of Proanthocyanidins in Black and Red Peanut Skins * DU Lei, LI Xin-hua (College of Food Science, Shenyang Agricultural University, Shenyang 110866, China) Abstract: The composition of proanthocyanidins of black and red peanut skins was investigated in this study. The pigment compounds in the skins of black and red peanuts were extracted with ethanol, separated and purified by organic solvent extraction and macroporous resin chromatography. The contents of proanthocyanidins were determined by high performance liquid chromatography (HPLC) and their composition was qualitatively analyzed by HPLC-mass spectrometry (HPLC-MS). The contents of proanthocyanidins in black and red pigments of peanut skins accounted for 29.19% and 43.04% of total proanthocyanidins, respectively. Four dimmers and 4 trimers of proanthocyanidins were identified in red peanut skin while 3 dimmers and 4 trimers of proanthocyanidins were identified in black peanut skin. This method was simple and fast and could be used for quantitative and qualitative analysis of proanthocyanidins in peanut skins. Key words: proanthocyanidins; high performance liquid chromatography (HPLC); HPLC-mass spectrometry; black peanut skin; red peanut skin