不同粒径及表面修饰的氧化铁纳米粒子的标准组合遗传毒性研究.pdfVIP

不同粒径及表面修饰的氧化铁纳米粒子的标准组合遗传毒性研究 1,2 1 1 1 1,2* 1,2* 刘燕萍 ，夏祺悦 ，杨润芳 ，刘强强 ，李宏霞 ，肖凯 （1. 四川大学华西医院国家成都中药安全性评价中心，四川 成都 610041 ） （2. 成都华西海圻医药科技有限公司，四川 成都 610041 ） 摘要：分别对以非功能性聚乙二醇 (SMG-10、SMG-25 和 SMG-30) 和表面带正电的聚醚酰亚胺 (SEI-10)修饰的粒径为10 - 30nm 的氧化铁纳米粒子进行标准组合遗传毒性（细菌回复突变试验、染 色体畸变试验、微核试验）研究。①Ames 试验中测试菌株采用 TA97、TA98、TA100、TA102 和 TA1535， 结果显示：SEI-10在加与不加 S9代谢活化系统下，都不会引起菌落回变增加；SMG-10在加与不加 S9代谢活化系统下，SMG-25仅在加 S9代谢活化系统下引起菌落回变增加。②SMG-10、SMG-30 和 SEI-10不会引起中国仓鼠肺细胞（CHL ）的染色体畸变率增加。③静脉注射 SMG-10、SMG-30 和 SEI-10后，小鼠骨髓嗜多染红细胞中的微核率与阴性对照组比较没有显著性差异。这些结果是对氧 化铁纳米粒子在其可能的医疗应用 （如诊断、治疗和药物靶向递送）领域中有关安全知识的有用扩 展。 关键词：表面修饰氧化铁纳米颗粒，细菌回复突变试验，遗传毒性，染色体畸变试验，微核试验 INTROCUTION In recent years, magnetic nanoparticles (MNPs) have generated significant interest within the scientific community due to their huge range of potential applications such as media materials for storage systems, biotechnology, magnetic separation targeted drug deliveryand vehicles for gene and drug delivery. Among the various MNPs, undoped and transition metal-doped, e.g., Magnetite or Maghemite, iron oxide nanoparticles, Fe O , γ-Fe O and M Fe O have found many applications in the area of biomedical 3 4 2 3 x y z diagnostics and therapy. （Bikram M, 2012. ）The increasing production and use of nanoparticles (NPs), has given rise to many concerns and debates among public, scientific and regulatory authorities regarding their safety and final fate in biological systems. Because of their very small size, NPs are capable of entering the human body by inhalation, ingestion, skin penetration o