家蚕抗热性分子检测研究.pdfVIP

国蚕学会第七届青年学术研讨会论文集 家蚕抗热性分子检测研究 刘彬斌 刘俊凤 陈义安 四川农业科学院蚕业研究所，四川南充 637000 摘要：利用生物信息学分析方法和实时定量PCR （Real Time PCR ）技术，对家蚕(Bombyx mori)低分子量热激蛋白基 因Bmhsp24.3进行了定量表达分析。家蚕基因芯片数据发现该基因在家蚕的体壁，脂肪体以及丝腺组织 有较高水平 表达，在其它组织中的表达不明显。Real Time PCR结果显示目的基因Bmhsp24.3在不同品种家蚕幼虫 都有所表达， 但在不同品种间的表达差异很明显。目的基因Bmhsp24.3在不同品种间的表达差异，刚好与品种的抗热性保持一致， 本研究实验结果说明品种的抗热性与目的基因Bmhsp24.3表达量存在正相关，目的基因Bmhsp24.3表达量越高，品种 的抗性越好；反之，目的基因Bmhsp24.3表达量越低，抗热性越差。 关键词：家蚕Bmhsp24.3、 抗热性、 实时定量PCR Heat resistance Study of Bombyx mori LIU Bin-Bin LIU Jun-Feng Du Zhou-He CHENG Yi-An Sericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong 637000, Sichuan Abstract: The expression of small heat shock gene Bmhsp24.3 of silkworm (Bombyx mori)was analyzed by bioinformatic analysis and Real Time PCR ．This gene Bmhsp24.3 expressed in skin, fat body and silk gland of silkworm. The expression level in other tissues are not Obvious. Real-time PCR data was used to reveal the gene expressed in larval tissues of Bombyx mori on day 3 of the fifth instar in different varieties, The expression level was remarkably difference in different varieties. The expression level ‘s difference in different varieties of Bombyx mori were in accordance with their heat resistance , Absolute quantization demonstrated that the varieties’ heat resistance of Bombyx mori was positive correlation with the gene ’s expression level . The higher of the purpose gene ’s expression level , the better of the varieties’ resistance. on the contrary, The lower of the purpose gene’s expression level , the poorer of the varieties’ resistance. Key words: Bmhsp24.3 Real Time PCR Heat resistance Bombyx mori 温室效应增强，全球性气温持续升高，使整个种养植业面临着高温的挑战。西部蚕区，夏秋蚕 期持续高温多湿是典型的气候特征，高温多湿的双重袭击将破坏蚕体各种生理机能的平衡，直接影 响蚕茧产量和质量