天生饮对肾血管性高血压大鼠肾Smad3和Smad7表达的影响.pdf

中文摘要 目的：研究天生饮对肾血管性高血压大鼠肾Smad3和Smad7表达的影响。 方法：将清洁级SD大鼠随机分成正常组、假手术组、手术组。实验 大鼠适应性喂养一周，采用二肾—夹法复制肾血管性高血压大鼠模型， 手术组均在无菌条件下进行左侧肾动脉夹窄术，假手术组只分离左侧肾 动脉不夹，正常组不作任何处理。术后第 4周，将造模成功的手术组大 鼠随机分为模型组和天生饮组，天生饮组每天给予天生饮灌胃，模型组、 假手术组和正常组以蒸馏水灌胃，连续灌胃四周后处死所有动物，记录 术前、术后至第 8周连续九次测压结果。处死动物时收集血液送生化实 验室检测 BUN 和 CR，同时用HE 方法观察左肾组织形态学改变，用免疫 组化学的方法分别检测大鼠左肾组织中Smad3和Smad7的表达。 结果：模型组大鼠与正常组、假手术组比较：血压、BUN及CR升高， 肾纤维化程度增加，Smad3的表达量上调，Smad7的表达量下调，以上组 间的差异均具有显著统计学意义 （P0.01）；经天生饮治疗四周后，天 生饮组大鼠与模型组比较：血压有所降低，但差异无统计学意义 （P＞ 0.05），BUN、CR降低，差异具有统计学意义 （P 0.01），肾纤维化程 度减轻，Smad3的表达量下调，Smad7的表达量上调，组间差异具有统计 学意义 （P 0.01）。 结论：天生饮能够减轻两肾—夹肾血管性高血压大鼠肾纤维化程度， 且其疗效机理可能是通过降低Smad3的表达和促进Smad7的表达。 关键词：天生饮；肾血管性高血压；Smad3；Smad7；肾纤维化 Effects of Tianshengyin on the Expressions of Smad3 and Smad7 in the Kidneys of Renovascular Hypertensive Rats ABSTRACT AIMS:To investigate the effect of Tianshengyin on the expressions of Smad3 and Smad7 in the kidney of renovascular hypertensive rats. METHODS: Sprague-Dawley(SD) rats with clean class were randomly subdivided into normal , sham-operated and operated group. After one week adaptability feeding, the rats were copied with two-kidney one clip(2K1C). In the operated group for renovascular hypertension, the rats underwent the operation via nipping the left renal artery. In the sham-operated group,the rats underwent the operation with only separating the left renal artery. Meanwhile there were no management on the rats in the normal group. Four weeks later, in the operated group, the rats with more than 150mmHg systolic blood pressure were randomly subdivided into model group and Tianshengyin group .The rats in Tianshengyin group were fed with Tianshengyin,While the ones in model group, sham-operated and normal group were fed with equal volume of distilled water respectively. After treat