5-氨基乙酰丙酸对中华稻蝗(Oxyachinensis)的杀虫活性及对3种酶活性的影响.docVIP

5-氨基乙酰丙酸对中华稻蝗（Oxya chinensis） 的杀虫活性及对3种酶活性的影响 阴 琨，马恩波，薛春荣，吴海花，郭亚平，张建珍 （山西大学生命科学与技术学院，太原 030006） 摘要：【目的】研究5-氨基乙酰丙酸（5-aminolevulinic acid，ALA）对中华稻蝗（Oxya chinensis）的杀虫活性及对3种酶活性的影响。【方法】以中华稻蝗4龄若虫为试验材料，用不同剂量的ALA（A1：250 mmol·L-1；A2：450 mmol·L-1；A3：750 mmol·L-1；A4：1 000 mmol·L-1）处理中华稻蝗，观察其对中华稻蝗的毒性效应和对其体内乙酰胆碱酯酶（AChE）、谷胱甘肽S-转移酶（GSTs）和谷胱甘肽过氧化物酶（GPx）活性的影响。【结果】不同剂量ALA处理组中华稻蝗死亡率依处理剂量呈现上升趋势，高浓度处理组A3、A4的死亡率分别达到66.19%和80.21%；LD50值为3.61（3.29～3.93）mg·g-1虫重（95%置信范围）。生化研究结果显示，最高浓度A4处理组雌、雄虫体内AChE活性分别较对照组下降了51.53%和42.65%，差异显著（P＜0.05）；GPx活性分别较对照组下降了42.82%和43.85%，差异显著（P＜0.05）。同时，中华稻蝗GSTs活性随ALA处理剂量升高而增高，A4处理组雌、雄虫体内GSTs活性分别较对照组升高了171.05%及97.42%，差异显著（P＜0.05）。【结论】ALA对雌、雄中华稻蝗均有明显的毒性效应；ALA可引起AChE和GPx光失活，从而导致中华稻蝗神经传导受阻同时抵御氧化损伤的能力下降；高剂量ALA激活GSTs，可引发昆虫对光毒性物质的自身反馈抵御反应。 关键词：中华稻蝗；5-氨基乙酰丙酸；乙酰胆碱酯酶；谷胱甘肽S-转移酶；谷胱甘肽过氧化物酶 Insecticidal Activities of 5-Aminolevulinic Acid on Oxya chinensis and Effect on Three Kinds of Enzymes YIN Kun, MA En-bo, XUE Chun-rong, WU Hai-hua, GUO Ya-ping, ZHANG Jian-zhen (School of Life Sciences and Technology, Shanxi University, Taiyuan 030006) Abstract:【Objective】Insecticidal activities of 5-aminolevulinic acid (ALA) on Oxya chinensis and the effect on activities of three enzymes were studied.【Method】 Fourth-instar nymphs of O. chinensis were treated with different doses of ALA (A1: 250 mmol·L-1; A2: 450 mmol·L-1; A3:750 mmol·L-1; A4: 1 000 mmol·L-1). Motality and the activities of acetylcholinesterase (AChE), glutathione S-transferase (GSTs) and glutathione peroxidase (GPx) were determinated.【Result】The mortality of O. chinensis increased with increasing dose of ALA. The mortality of high-dose treatments A3 and A4 reached 66.19% and 80.21%, respectively. The value of LD50 was 3.61 (3.29-3.93) mg·g-1body weight (95% confidence interval). Biochemical studies showed that the activities of AChE and GPx in A4 treatment declined by 51.53% and 42.82% in female and 42.65% and 43.85% in male compared to control, respectively, and the degree of decline reached significant