16S_23SrRNAITS_AFLP指纹_省略_分析在白酒窖泥原核微生物多样性.pdfVIP

年第 期（总第 期）· ) 46 酿酒科技 2010 3 189 LIQUOR－MAKING SCIENCE ＆ TECHNOLOGY 2010 No．3(Tol．189 16S-23S rRNA ITS-AFLP 指纹图谱分析在白酒窖泥 原核微生物多样性分析中的应用 邓 依，唐云容，张文学 （四川大学轻纺与食品学院，四川 成都 610065 ） 摘 要： 利用非培养的分子生物学方法对位于多粮原料生产的窖壁和窖底的新窖和老窖窖泥中原核微生物的 16S-23S rRNA ITS 进行AFLP(amplified fragment length polymorphism)分析，利用NTSYSpc 2.10e 软件构建聚类图， 分析其关系。结果表明，来自不同窖池的不同部位的原核微生物在16S-23S rRNA ITS 水平上呈现出差异，其中老窖 窖底和新窖窖底的原核微生物多样性存在着明显差异，其相关系数为0.47 ；而老窖窖壁和新窖窖壁的原核微生物多 样性却差异不大，其相关系数为1.00。 关键词：白酒； 窖泥； 原核微生物； 16S-23S rRNA ITS-AFLP ： ； ； ： ： （ ） 中图分类号 TS262.3 TS261.4 Q93-3 文献标识码 B 文章编号 1001－9286 2010 03－0046－02 Application of 16S-23S rRNA ITS-AFLP Fingerprint in the Analysis of Prokaryotic Microbial Diversity in Liquor Pit Mud DENG Yi, TANG Yun-rong and ZHANG Wen-xue (Light Textile Food Science College of Sichuan University, Chengdu, Sichuan 610065, China) Abstract: 16S-23S rRNA ITS-AFLP (amplified fragment length polymorphism) analysis of prokaryotic microbes in pit mud of new pits and aged pits was operated by use of non-culture molecular biological method. Then the cluster diagrams were established by NTSYSpc 2.10e software to study the relationship among microbes. The results showed that prokaryotic microbes varied at different part in different pits at 16S-23S rRNA ITS level. There was evident difference in prokaryotic microbial diversity between aged pit bottom mud and new pit bottom mud (the coefficient was 0.47), however, such difference was little between aged pit wall mud and new pit wall mud (the coefficient was 1.00).