Annexin+V-FITC2fPI法检测脂多糖诱导滋养细胞的凋亡.pdfVIP

of Medical 沈阳医学院学报Journal Shenyang Annexin V—FITC／PI法检测脂多糖诱导滋养细胞的凋亡 李丹妮1，吴婷2，邹黎明1 (1．沈阳医学院基础医学院生物化学教研室，辽宁沈阳110034；2．厦门市妇幼保健院产科) V-FITC／PI 胞凋亡的关系，为预防和治疗由滋养细胞凋亡导致的病理妊娠提供新的思路。方法：利用流式细胞仪Annexin 法检测不同浓度的LPS处理培养的JEG-3细胞系滋养细胞的凋亡率分析。结果：流式细胞检测结果显示LPS组凋亡指数分 别为(2．054-0．33)％、(7．43±0．68)％、(13．434-0．90)％显著高于对照组(0．804-0．15)％，且P均0．01。结论： Annexin V．FITC／PI法能特异地、准确地检出早期凋亡的细胞、继发性坏死的细胞；LPS能够诱导滋养细胞过度凋亡，且凋 亡率随着LPS浓度的增加而增加。 V-FITC／PI [关键词]LPS；凋亡；流式细胞术；Annexin [中图分类号]R329．2[文献标识码]A [文章编号]1008—2344(2008)03—0142—03 DetectionaboutLPS-inducedof Flow fAnnexinV·F兀C／PI ApoptosisCytotrophoblastsby Cytometry Methods) Staining LIDan．ni 1。WU Tin92。ZOULi．ruin91 of Medical 1 of andChildHealthHos— (1．DepartmentBiochemistry，ShenyangCollege，Shenyang10034．China；2．DepartmentObstetrics，Maternal of pitalXiamen) the betweenLPSand andto newlineof [Abstract]Objective：To identifyrelationship cytotrophoblasticapoptosisprovide and of caused effectof forthe treatment thought prevention pathologicalpregnancybycytotrophoblastieapoptosis，thelipopolysaecha- rideU)Son w∞detectedflow thetreatmentofJEG．3 eytotrophoblasticapoptosis by cytometry(FCM)．Methods：Aftertrophoblasts withthedifferentconcentrationsof indexof weredeterminedand V- LPS，theapoptosiscytotrophoblasts analysizedbyFCM(Annexin FITC／PI resultsfromFCMdemonstratedthatthe indexofLPS were SminingMethods)．Results：The apoptosis group respectively