水母雪莲红色细胞系类黄酮含量和相关基因表达.pdfVIP

圜 ISSNi000．3061CN11-1998Q／ChinJBi。teecchAugust25，2014Vo1．30No．8 Flavonoidscontentsandexpressionanalysisofrelated genesinredcelllineofSaussureamedusa YajieWang，HouhuaLi，WanyiFu，YanGao，BingjieWang，andLingLi CollegeofForestry，NorthwestAFUniversity，Yangling712100，Shaanxi，China Abstract： Saussurea medusa iSararetraditionalChinesemedicinalherb．Besidesanti-inflammatory and analgesic activities，ithaseffectsofdisinhibitingcold，dispellingdampnessandpromotingbloodcirculation．Flavonoidsarethemain medicinalcompoundsin medusa．Contentsofflavonoidsandexpressionofflavonoidsbiosynthesisrelatedgenesinwhite andred(inducedbylowtemperature，highSUCrOSeandhighlight)calluswereanalyzed．Theresultsshowedthatthetotal flavoneinred linewas3．60timeshighercomparedtowhiteline．Theaccumulationofrutininredlinef0．25％ ofdry weight)was2．40timeshighercomparedtowhiteline．Anthocyaninswereabundantinredline．withthecontentsof cyanidin3-O—glucosidech1orideandcyanidin3-O-succinylglycoside0．12％ and0．19％ ofdry weightrespectively．CHS, F3tH，FNS FLs，DFR andANS geneswerehighly expressed in red line comparedtowhiteline．Expression ofthree transcriptionfactors(MYB，bHLHandWD40)inredlinewassignificantlyhigherthanthatinwhiteline，especiallythe expressionofMYB (19．70timeshighercomparedtowhiteline)．Theseresultsindicatedthathighexpressionlevelsof transcriptionfactorsinducedhighexpressionofstructuralgenesinredline，therebyenhancingtheflavonoidsbiosynthesis． TheexpressionofbHLH andWD40wassimilar,whereasitwassignificantlydifferentfrom thatofMYB，indicatingthat bHLH andW D40couldforillabinary complextoregulateexpressionofstructuralgenesandflavonoidsbiosynthesis． Keywords： Saussureamedusa，transcriptionfactorscomplex，structuralgenes，flavonoidsbiosynthesis，expressionanalysis 水母雪莲 SaussureamedusaMaxim是多年 酶 A，香豆酰辅酶 A来源于苯丙素代谢途