Chloroplast Proteomics and the Compartmentation of Plastidial Isoprenoid Biosynthetic Pathways.pdfVIP
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Chloroplast Proteomics and the Compartmentation of Plastidial Isoprenoid Biosynthetic Pathways.pdf
MolecularPlant ·Volume2 ·Number6 ·Pages1154_1180 ·November2009 REVIEW ARTICLE
ChloroplastProteomicsandthe
COmpartmentatiOnofPlastidialIsoprenoid
BiosyntheticPathways
JacquesJoyard,Myriam Ferro,ChristopheMasselon ,Daphn~Seigneurin-Berny,DanielSalvi,
J~r6meGarinbandNorbertRolland。,’
a LaboratoiredePhysiologieCellulaireVeg6tale;UMR5168;CEA;CNRS;INRA;Universit~JosephFourier;iRTSV,CEA·Grenoble.38054Grenoble’cedex9,France
b LaboratoireEtudedelaDynamiquedesProt~omes;U880;CEA;INSERM;Universit~JosephFourrier;iRTS~ CEA—Grenoble,38054Grenoble—cedex9,France
ABSTRACT Recentadvancesjntheproteomicfieldhaveallowedhigh—throughputexperimentstobeconductedonchlo。
roplastsamples.Manyproteomicinvestigationshavefocusedoneitherwholechloroplastorsub-plastidialfractions.To
date,thePlantProteinDatabase(PPDB,Suneta1.,2009)presentsthemostexhaustivechloroplastproteomeavailable
online.However,theaccurateIocalizationofmanyproteinsthatwereidentifiedindi仟erentsub—plastidialcompartments
remainshypothetica1.Ferroeta1.(2009)wentastepfurtherintotheknowledgeofArabidopsisthalianachloroplastpro-
teinswithregardstotheiraccurateIocalizationwithinthechloroplastbyusingasemi-quantitativeproteomicapproach
knownasspectralcounting.TheirproteomicstrategywasbasedontheaccuratemassandtimetagsA【MT)database
approachandtheybuiltupATCHLOR0,acomprehensivechloroplastpr6teomedatabasewithsub-plastidiallocalization
andcuratedinformationonenvelopeproteins.Comparingthesetwoextensivedatabases,wefocushereonabout100
enzymesinvolvedinthesynthesisofchloroplast-specificisoprenoids.Wellknownpathways(i。e.compartmentationofthe
methylerythritolphosphatebiosyntheticpathway,oftetrapyrrolesandchlorophyllbiosynthesisandbreakdownwithin
chloroplasts)validatethespectralcounting—basedstrategy.Thesamestrategywasthenusedtoidentifytheprecise
IocalizationofthebiosynthesisofcarotenoidsandprenyIquinoneswithinchloroplasts(i.e.inenvelopemembranes,
stroma,and/orthylakoids)thatremainsunclear
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