甲状腺素对心房和肺静脉神经和组织重构的影响.docVIP

甲状腺素对心房和肺静脉神经和组织重构的影响 李华康1，殷跃辉2,宋治远2 （400038 重庆，第三军医大学西南医院心血管内科，重庆市介入心脏病学研究所tyrosine hydroxylase，TH）rowth associated protein-43，GAP-43）在心房和肺静脉中的表达，从而探讨甲状腺素对心房和肺静脉组织及神经重构的影响。方法 新西兰白兔20只，随机分为实验组和对照组，每组各10只。实验组腹腔注射左旋甲状腺素(levothyroxine L-Thy 50μg/kg/d×28d)建立甲亢动物模型。分别于实验第0、7、14、21、28天测定兔心率、体质量、体温，耳缘静脉采血，用放免法测TT3、TT4？。于实验第28天收集心房后壁和肺静脉组织，光镜下观察心房、肺静脉形态结构，测定心肌细胞直径，Van Gieson（VG）染色测定胶原容积分数（CVF），免疫组化半定量检测GAP-43在左、右心房及肺静脉中的表达,RT-PCR半定量检测TH、GAP-43 mRNA在左、右心房和肺静脉中的表达。结果 实验组与对照组相比，心房肌细胞直径增加[左心房(9.58±0.57)vs(4.79±0.31)；右心房 (8.19±0.65)vs (4.67±0.29)]、心肌胶原容积分数升高[左心房 (0.36±0.06) vs (6.71±0.11)；肺静脉(0.19±0.01) vs (4.29±0.12)；右心房(0.3±0.12) vs (4.62±0.4)]；实验组左、右心房及肺静脉GAP-43阳性表达较对照组明显增加（P＜0.01）；实验组左、右心房及肺静脉GAP-43mRNA和THmRNA表达较对照组增加（P＜0.01）。结论 L-Thy可诱导心肌细胞结构改变、心肌间质纤维化以及心房和肺静脉交感神经增生。 [关键词] 甲状腺素；神经重构；TH；GAP-43 [中图法分类号] [文献标志码] A The effect of thyroid hormone on nerve and histological remodeling of pulmonary vein and cardiac atrium Li Huakang1，Yin Yuehui2，Song Zhiyuan1(Department of Cardiology，Second Affiliated Hospital of Chongqing University of Medical Sciences，Chongqing 400010，China.Abstract] Objective To study the expression of Tyrosine hydroxylase（TH） and growth associated protein（GAP-43）in the cardiac atrium and pulmonary vein and to explore effect of thyroid hormone on nerve and histological remodeling of pulmonary vein and cardiac atrium. Methods Twenty New Zealand rabbits were randomly divided into two groups: control group (n=10) and experimental group(n=10). Hyperthyroidism animal model was established by peritoneal injection of Levothyroxine (L-Thy). All rabbits were followed up for 4 weeks. The heart rate, body weight,body temperature and blood samples were obtained at baseline and 7 days interval. Serum TT3 and TT4 were measured with radioimmunoassays (RIAs). Posterior wall of the cardiac atrium and pulmonary vein tissues were collected at the end of the 4th weeks. cardiomyocyte diameter, structural change were detected. Ca