the苄嘧磺隆对蛋白核小球藻的生长效应研究guidedownload.pdfVIP

中国农业科学 2006,39(9):1823-1827 Scientia Agricultura Sinica 苄嘧磺隆对蛋白核小球藻的生长效应研究 岳霞丽，张新萍，胡先文，董元彦 （华中农业大学理学院，武汉 430070 ） 摘要：【目的】研究苄嘧磺隆对蛋白核小球藻（Chlorella pyrenoidosa ）的生长效应，评价苄嘧磺隆的生态 风险。【方法】通过不同浓度的苄嘧磺隆对蛋白核小球藻的 96 h 急性毒性试验，研究苄嘧磺隆对小球藻的生长及 藻细胞中叶绿素和蛋白质含量的影响。【结果】低浓度苄嘧磺隆（＜1 mg·L-1 ）具有刺激藻细胞生长的作用，其叶 绿素含量和蛋白质含量均有明显的增加；高浓度的苄嘧磺隆(＞5 mg·L-1)抑制藻的生长，藻细胞叶绿素含量和可溶 性蛋白质含量随药剂浓度增加而明显下降，且表现出较好的计量－效应关系，其对蛋白核小球藻的 96h-EC50 值为 15.7 mg·L-1 。【结论】苄嘧磺隆对蛋白核小球藻的生长有一定的抑制作用，属低毒。 关键词：苄嘧磺隆；蛋白核小球藻；生长；叶绿素；蛋白质 Effect of Bensulfuron-Methyl on Growth of Chlorella pyrenoidosa YUE Xia-li, ZHANG Xin-ping, HU Xian-wen, DONG Yuan-yan (College of Basic Science, Huazhong Agricultural University, Wuhan 430070) Abstract ：【Objective 】To study the growth effects of differing concentrations of Bensulfuron-Methyl on Chlorella pyrenoidosa and to evaluate the ecological risk. 【Method 】The effects of Bensulfuron-Methyl on the growth and the content change of chlorophyll and protein in Chlorella pyrenoidosa were studied through 96 h acute toxicity tests. 【Result 】Bensulfuron-Methyl accelerated the growth of algae at a lower concentration (1 mg·L-1) with content increase of chlorophyll or protein, and it inhibited the growth of algae at higher concentration (5 mg·L-1). The content of chlorophyll or protein in algae cells reduced with the increasing concentration of Bensulfuron-Methyl, exhibiting the good concentration-effect relationship. The 96h-EC50 of Bensulfuron-Methyl upon the algae was 15.7 mg·L-1. 【Conclusion 】Bensulfuron-Methyl has inhibiting effect on the growth of Chlorella pyrenoidosa and is lower toxicity. Key words：Bensulfuron-Methyl; Chlorella pyrenoidosa; Growth; Chlorophyll; Protein