两性霉素B聚氰基丙烯酸正丁酯纳米粒的制备及小鼠体内分布研究.pdfVIP

242 2007 M ar; 28( 3) Acad J Sec Mil Med U niv 论 著 B 1 2 3 1 1* 徐 楠 , 邹 豪 , 范国荣 , 温 海 , 陈江汉 ( 1., 200003;2., 200433; 3. , 200433) [ ] 目的: 制备携载两性霉素的聚氰基丙烯酸正 丁酯纳米粒(Am P CANP) , 研究其对血脑屏障的透过 力方法: 孵化法制备Am P CANP, 以聚山梨酯80 进行表面修饰, 建立高效液相色谱分析法, 流动相选择乙腈水(40 60) 及4% 乙 酸,405 nm 处检测小鼠分 3 组, 每组28 只, 分别注射Am 粉针剂脂质体( Am L) 及纳米制剂( Am P CANP) , 通过检测 小鼠脑组织等脏器中的药物浓度, 评价其主动脑靶向作用结果: Am P CANP 平均粒径94. 38 nm, 平均包封率为 82% , 载药量56. 1% 给药后Am 粉针剂组小鼠脑内未 检测出药物,Am L 组于3 h 后测得微量浓度, 而Am P CANP 组小 鼠30 min 即测得可观浓度, 3 h 后达133 ng/ g结论: 聚山梨酯80 修饰的Am P CANP 具有主动脑靶向作用 [ ] 脑膜炎, 隐球菌性; 两性霉素 ; 聚氰基丙烯酸正 丁酯; 血脑屏障 [ ] R 978. 5 [ ] A [ ] 0258879X(2007 Preparation of amphotericinBpolybutylcyanoacrylateloadednanoparticlesanditsdistributioninmice 1 2 3 1 1* XU Nan , ZOU Hao , FAN Guorong , WEN Hai , CHEN Jianghan ( 1. Department of Dermatology, Changzheng Hospital, Second Military M edical University, Shanghai 200003, China; 2. Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433 ; 3. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military M edical University, Shanghai 200433) [ABSTRACT] Objective:T o prepare amphotericin polybutylcyanoacrylateloaded nanoparticle ( Am P CANP) and to assess their ability to pass the bloodbrain barrier. ethods: Am P CANP was prepared by incubation, w ith the surface modified by polysorbate 80. Highperformance liquid chromatography ( HPLC) method was established using acetonitrile and water (40 60, 4% of acetic acid) as mobile phase; detection w as done at 405 nm. M ice w ere divided into 3