蛋白质组技术与应用20131210.pdf

An Introduction to Proteomics 闻博 2013-12-09 Outline  The basic concepts of proteomics  Mass Spectrometry: Concepts and Components  MS-based protein identification strategy  Quantitative proteomics Proteome and Proteomics The analysis of the entire PROTEin complement expressed by a genOME, or by a cell or tissue type. Marc Wilkins in: Wasinger VC et al, Electrophoresis 16 (1995) “PROTEOMICS is the systematic analysis and documentation of proteins in biological samples with focus on state-related expression of proteins.” Stems from “PROTEin ”and “genOMICS ” 3 Why Proteomics? • Proteomics grew out of Genomics – Whole Genome Sequence –complete, but does not show how proteins function or biological processes occur – Level of mRNA not necessarily equal to level of protein product – Some transcripts give rise to multiple products (alternative splicing, etc) – Gain insight into alternative splicing – Aids in genome annotation • Proteins are the actuators: – Direct measurement of protein = best measurement – Many undergo post-translational modifications–proteins sometimes chemically modified or regulated after synthesis – Proteins fold into specific 3-D structures which determine function Proteomics Applications  Scientific Knowledge - Proteomics has been used to study basic biological questions. For example, what is the structure of the pores between the nucleus and the cytoplasm of a cell? Diagnosis - Proteomics has looked at tumor biopsies and bloo