蛋白质组生物信息学分析.pdfVIP

Bioinformatics for Proteome 武汉金开瑞生物工程有限公司 OUTLINE Summary of Proteome Bottleneck in Proteome based on MS/MS Qualitative Proteome Analysis Quantitative Proteome Analysis Post Translational Modifications(PTMs) Functional Annotation of large scale protein data set Summary of proteome proteomics Summary of proteome proteomics Bottleneck in Proteome based on MS/MS Reproducibility Quantification Accuracy Reliability Identification Identification of workflow How to identify a protein/pep seq. Raw data from mass spectrum MS spectra: Peptide ions （precursors, mother ions） MS/MS spectra: fragment ions （product ions） example.mgf Charge of precursor ion Mass of precursor ion Peptide fragment ions： m/z intensity charge End symbol of one MS/MS spectrum Peptide ion fragmentation Available search engines Why ProteinPilot • Paragon™ algorithm: ‘Sequence approach’ search algorithm for peptide ID; “The Paragon Algorithm, a Next Generation Search Engine That Uses Sequence Temperature Values and Feature Probabilities to Identify Peptides from Tandem Mass Spectra” Shilov IV, Seymour SL et al (2007) MCP 6.9, 1638 • Unique feature • hypothesis selection stage “… there is greater potential for improvement from advances in determining what to score, not how to score it.” Shilov IV, Seymour SL et al (2007) MCP 6.9, 1638 • ProGroup™ algorithm for protein inference • Quant