含离子液体介质中脂肪酶高效催化对羟基苯甘氨酸甲酯不对称氨解_吴虹.pdfVIP

21 3 No.3 Vol.21 2007 6 Journal of Chemical Engineering of Chinese Universities June 2007 1003-9015 (2007)03-0481-07 , , ( , 510640) C MIm·BF (20 v/v)/Novozym 435 6 4 D-120~160 mmol⋅L− 1 pH 0.55~0.7535~40 7.5~8.0 40 mmol⋅L− 1 5.88 mmol⋅L− 1⋅h− 1 83 (E )6 h47.6 e.e. 92.9 Novozym 435 C MIm·BF (20 v/v)/ 6 4 7 90 41 TQ925.6 TQ225.41 A Asymmetric Ammonolysis of D,L-p -hydroxyphenylglycine Methyl Ester Catalyzed by Lipase in an Ionic-Liquid-Containing System WU Hong, LOU Wen-yong, ZONG Min-hua (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) Abstract: Using lipase Novozym 435 as mediator and the ionic liquid (IL), C6MIm·BF4 (20%, v/v)/ terrt-butanol co-solvent mixture, as reaction medium, the enantiomerically pure D-p-hydroxyphenylglycine amide was prepared via asymmetric ammonolysis of D,L-p-hydroxyphenylglycine methyl ester (D,L-HPGME). Ammonium carbamate was found to be the best ammonia donor for the reaction and its favorable concentration is 120~160 mmol⋅L− 1. The optimal water activity, reaction temperature, pH value and concentration of D,L-HPGME are 0.55~0.75, 35~40 , 7.5~8.0 and 40 mmol⋅L− 1, respectively. Under above mentioned conditions, the initial reaction rate and enantiomeric ratio (E) of the reaction are 5.88 mmol⋅L− 1⋅h− 1 and 83. After reaction time of 6 h, the yield and the product e.e. are 47.6% and 92.9%, respectively. Comparing with using terrt-butanol as reactio