低剂量电离辐射对DNA拓朴异构酶Ⅱα表达作用.pdfVIP

http :// Effect of Low Dose Ionizing Radiation on the Expression of DNA Topoisomerase Ⅱα Wen Liu, Hua Li, Jinyan Li, Ji Zuo* Department of Cellular and Genetic Medicine Shanghai Medical College Fudan University, Shanghai, PRC 200032 *Correspondence to: Prof . Dr. Ji Zuo j zuo@ liuwen@ Granted by National Natural Science Fundation of China Abstract DNA topoisomerase (TOPO ) is a nuclear enzyme involved in chromosome segregation and other essential cellular processes. IHC and Western blot were used to determined the changes of TOPO II a expression and of its behavior in HeLa cells irradiated withγ-rays(≤2 Gy). A tran sient and significant decrease of TOPO after low doseγ-rays exp osure was detected: the decrease began at 4h after irradiation , reached to the lowest level at 12h, and began to return at 16h. Given low dose γ-rays, the level of decrease added as dose was added. The level of decrease after multiple irradiation was higher than that after single irradiation. This observation indicates that the decrease of TOPO afterγ-rays is