花青素分光光度法测定铝.docVIP

天然显色剂花青素体系 在光度法测定铁中的应用 摘 要：研究了自紫甘蓝中提取的花青素与Fe3+发生显色反应的条件，建立了一种测定Fe3+的方法。在g·L-1溴化十六烷基三甲基的存在下，花青素在pH 4.7的乙酸-乙酸钠缓冲体系中与Fe3+形成三元胶束络合物显色，络合物最大吸收波长620nm。Fe3+含量在～0 mg·L-1范围内与吸光度呈良好的线性关系，相关系数为0.9994，络合物摩尔吸光系数2.0×103 L·mol-1·cm-1，Fe3+浓度为25 mg·L-1时方法相对标准偏差1.84%，检出限0.92mg·L-1。方法应用于国家岩矿标准样品（超基性岩DZE-2）的测定获得准确结果，岩矿品加标回收率在95.6% ~108%之间。方法可用于低常量微量铁样品的测定。 关键词：花青素；铁；；分光光度法中图分类号：O657.3atural colour-display reagent anthocyanins- cetyltrimethylammonium bromide system in the application of spectrophotometric determination of Ferrum LIU Jian, HU Xiao-rong, and LI Ting (College of material and chemistry chemical engineering, Chendu university of technology, 610059 ) Abstract: A method of the quantitative determination of Fe3+ was created basing on the different color between anthocyanin itself extracted from the red cabbage and the product of the complexation that Fe3+ reacting to anthocyanin and cetyltrimethylammonium bromide(CTMAB). In the presence of 0.42 g·L-1 CTMAB, Anthocyanins colored with Fe3+ in acetic acid - sodium acetate buffer at pH 4.7. The maximum absorption wavelength of the complex was 620nm. There was well linear relationship between absorbance and Fe3+ of which content was in the range of 4.0～0 mg·L-1, and correlation coefficient was 0.9994. The molar absorption coefficient of complex was 2.0×103 L· mol-1·cm-1. The precision of replicate measurements was 1.84% at Fe3+ concentrations of 25mg·L-1. A quantitative solution detection limit of 0.92mg·L-1 was obtained. The analytical procedure was critically evaluated by analyzing certified rock reference materials (super mafic rocks DZE-2) and determining the recovery of Fe3+ in the materials. Excellent agreement between the experimental values and the certified concentrations and the optimum recoveries of 95.6% ~108% by addition of the standard solution in the rock reference materials was obtained. This method can be used for the quantitative determination of trace amounts and low levels of Ferrum. Key words: Anthocyanins