PCR_DGGE的研究处理垃圾渗滤液序批式生物膜反应器_SBBR_中细菌多样性.pdfVIP

28 5 Vol. 28, No.5 2007 5 ENVIRONMENTAL SCIENCE May, 2007 PCR-DGGE (SBBR) 1 1* 1 2 1 1 1 , , , , , , ( 1, 410082; 2 , 100080) : , , COD SBBR DNA, ( GC341F907R) DNA 16S rDNA , 16S rDNA DGGE, , ., SBBR , , ; SBBR , 3 . SBBR . : 16S rDNA; SBBR;PCR; DGGE; ; : X172 :A :0250 3301( 2007) 05 1095 07 Bacterial Diversity in Sequencing Batch Biofilm Reactor ( SBBR) for Landfill Leachate reatment Using PCR DGGE 1 1 1 2 1 1 XIAO Yong , YANG Zhao hui , ZENG Guang ming , MA Yan he , LIU You sheng , WANG Rong juan , XU Zheng 1 yong ( 1College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; 2Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China) Abstract:For studying the bacterial diversity and the mechanism of denitrification in sequencing bath biofilm reactor ( SBBR) treating landfill leachate to provide microbial evidence for technique improvements, total microbial DNA was extracted from samples which were collected from natural landfill leachate and biofilm of a SBBR that could efficiently remove NH+ N and COD of high concentration. 16S rDNA fragments were 4 amplified from the total DNA successfully using a pair of universal bacterial 16S rDNA primer, GC341F and 907R, and then were used for denaturing gradient gel electrophoresis ( DGGE) analysis. The bands in the gel were analyzed by statistical methods and excided from the gel for sequencing, and the sequenceswere used for homology analysis and then two phylogenetic trees were constructed using DNAStar software. Results indicated that the bacterial diversity of the biofilm in SBBR and the landfill