β溶血型链球菌诱发点滴型银屑病发病机制的研究.x.docxVIP

β溶血型链球菌诱发点滴型银屑病发病机制的研究β溶血型链球菌诱发点滴型银屑病发病机制的研究中华皮肤科杂志 1999年第2期第32卷 论著作者：张理涛　陈学荣　殷金珠单位：100083 北京医科大学第三医院皮肤科；张理涛现在天津长征医院皮肤科；陈学荣　殷金珠北京医科大学临床免疫室关键词：银屑病；链球菌属【摘　要】　目的　探讨β溶血型链球菌所诱发的急性点滴型银屑病的发病机制。方法　应用MTT方法观察急性点滴型银屑病患者外周血淋巴细胞(PBMC)对链球菌抗原的反应，用3H-TdR掺入法和免疫组织化学方法检测链球菌抗原刺激的PBMC培养上清液对人类角质形成细胞株COLO-16DNA合成以及HLA-DR、ICAM-1分子表达的影响。结果　急性点滴型银屑病患者PBMC对链球菌抗原的反应较对照组更为明显(P＜0.01)，其24h链球菌抗原刺激 的PBMC培养上清液对角质形成细胞的促增殖作用较对照组显著增强(P＜0.01)，并能诱导角质形成细胞表达HLA-DR和ICAM-1。结论　具有银屑病素质的个体体内可能存在链球菌抗原特异性的T淋巴细胞，受链球菌抗原或超抗原作用后增殖，产生大量细胞因子，导致角质形成细胞过度增生和HLA-DR、ICAM-1异常表达。Study on Pathogenesis of Guttate Type Psoriasis Inducedby Group A β-haemolytic Streptococcal InfectionZHANG Litao,CHEN Xuerong,YIN Jinzhu.Department of Dermatology, The Third School of Clinical Medicine, Beijing Medical University, Beijing 100083【Abstract】　Objective　To investigate the pathogenesis of acute guttate type psoriasis (AGP) induced by streptococcal infection.Methods　The proliferative responses of peripheral blood mononuclear cells (PBMCs) to streptococcal antigens were detected by using MTT methods. Effects of supernatants of PBMCs cultured with streptococcal antigens on DNA synthesis of keratinocytes were measured by 3H-TdR incorporation assay,and immunohistochemical staining was used to examine HLA-DR and intercellular adhesion molecule 1 (ICAM-1) expression on keratinocytes.Results　The proliferative responses of PBMCs to streptococcal antigens were greatly enhanced in patients with AGP than those in controls (P＜0.01).The supernatants of PBMCs in patients with AGP stimulated by streptococcal antigens could significantly increase 3H-TdR incorporation with keratinocytes (P＜0.01).And these supernatants from patients with AGP could also induce the expression of HLA-DR and ICAM-1 in human keratinocytes.Conclusion　There might be streptococcal antigen-specific T lymphocytes in AGP patients.【Key words】　PsoriasisStreptococcusβ溶血型链球菌感染与急性点滴型银屑病(AGP)的相关性早已引起皮肤科医师的注意，但其发病机制不明。我们应用链球菌悬液作用于AGP患者的外周血淋巴细胞，了解其对链球菌抗原的反应；并且收获外周血淋巴细胞(PBMC)的24h培养上清液作用于人类角质形成细