舟山野生灵芝多糖制备工艺的优化研究【毕业论文】.doc

本科毕业论文 （20 届） 舟山野生灵芝多糖制备工艺的优化研究 专业：药学 目 录 1前言： 5 2仪器与材料 6 2.1实验仪器 6 2.2实验材料 7 3 实验方法 7 3.1样品预处理 7 3.2灵芝多糖的提取 7 3.3不同因素对灵芝多糖提取量的影响（单因素实验） 7 3.4制定灵芝多糖最佳制备工艺条件的正交试验 8 4结果与讨论 10 4.1不同因素对灵芝多糖提取量的影响 10 4.2正交试验灵芝多糖粗品得量 13 5结论 14 参考文献： 14 [摘要] 灵芝多糖是灵芝中最有效的成分之一，因此，也特别受到医药科技工作者的重视，研究报道也最多。现知灵芝多糖有广泛的药理活性，能提高机体免疫力，提高机体耐缺氧能力，消除自由基，抑制肿瘤、抗辐射，提高肝脏、骨髓、血液合成DNA、RNA、蛋白质能力，延长寿命，灵芝多糖还具有刺激宿主非特异性抗性、免疫特异反应以及抑制移植肿瘤生理活性的特性。多糖分子量大于1×104时显示强抑制肿瘤活性，活性强弱还与多糖链分叉的程度及支链上羟基的数量有关。 对心血管疾病、气喘、过敏、神经衰弱、胃热等有显著效果。还具有降血压、降血脂、解血瘀、改善血液循环、皮肤美容等作用。灵芝的多种药理活性大多和灵芝多糖有关。本试验利用微波技术研究了料液比、微波作用时间和微波功率对灵芝多糖提取率的影响规律，采用正交试验，得出灵芝 [Abstract]Ganoderma lucidum polysaccharides is one of the most effective ingredients, therefore, also particularly affected by medical science and technology workers seriously, study reports also the most. Ganoderma lucidum polysaccharides are known a wide range of pharmacological activity, can improve immunity, enhance the body hypoxia tolerance, elimination of free radicals, inhibit tumor, anti-radiation, the liver, bone marrow, blood synthesis of DNA, RNA, protein ability to prolong life, Ganoderma lucidum polysaccharides also have non-specific stimulation of host resistance, immune response and inhibition of specific physiological activities characteristic of tumor transplantation. Polysaccharide molecular weight greater than 1 × 104 to display strong inhibition of tumor activity, activity strength also with the degree of branching of polysaccharide chains and branched-chain related to the number of hydroxyl groups. For cardiovascular disease, asthma, allergies,neurasthenia, stomach heat such as a significant effect. Also have lower blood pressure, blood fat, blood stasis solutions, improve blood circulation, skin care and so on. Most of a variety of pharmacological activities of Ganoderma lucidum and Ganoderma lucidum polysaccharides on. The trial studied the use of microwave solid to liquid ratio, reaction time and microwave power of microwave Ganoderma lucidum polysaccharides on the rat