肠外致病性大肠埃希菌多重耐药性及氨基糖苷类耐药基因分析.pdfVIP

·498· 肠外致病性大肠埃希茵多重耐药性及氨基糖苷类耐药基因分析 张璇汤细彪 吴斌‘ 陈石 夏欣满晓营 陈焕春 (华中农业大学农业微生物学国家重点实验室； 武汉430070) 摘要： 目的 检测临床分离猪、鸡源肠外致病性大肠埃希菌的耐药性，分析其携带的氨基糖苷类耐药基因，以指导临床用药， 探究细菌对氨基糖苷类抗生素的抗性机制。方法应用K—B法测定分离株对19种常用抗生素的耐药情况。用双纸片法检测产 (3’)．Ia，aph(3 药基凶符合率高(≥74％)且序列保守。结论猪、鸡源肠外致病性大肠埃希菌呈多霞耐药性，其对氨基糖苷类抗生素的抗性机制以 产生针对该类抗生素的修饰酶为主。 关键词： 肠外致病性大肠埃希菌； 耐药性； 氨基糖苷类抗生素； 耐药基因型 中图分类号：R378．2+l文献标识码：A of resistanceandtheresistanceto Analysismulti-drug genes antibioticsofextraintestinalEschedchiacoli pathogenic Man andChenHuan—chun Xi-biao，WuBin，ChenShi，XiaXin， Xiao—ying ZhangXuan，Tang of 430070) (StateKeyLaboratoryAgriculturalMicrobiology，HuazhongA州culturalUniversity，Wuhan ABSTRACT To theresistancetocommonantibioticsandresistanceto Objectiveinvestigate genes antibioticsofextraintestinalEscherichia from and coli(ExPEC)isolated aminoglycoside pathogenic infecjedpigs chickens．Methodsr11IleresistanceofExPECisolatesto19kindsofantibioticsweredetected by diffusion isolatesweredetecteddouble-disc method．Extended—spectrum13-laetamases(ESBLs)producingby synergy testandwereclassified．PCRand were todetect designed sequencing Theseisolatesshowed resistance．ne of