蒙药诃子中多糖的提取及含量测定.pdfVIP

内蒙古医科大学学报摇 2014年8月摇 第36卷摇 第4期 ·339 · 蒙药诃子中多糖的提取及含量测定 于姝燕,摇 陈建平,摇 杜艳青,摇 代慧子,摇 生昊杨,摇 罗素琴* (内蒙古医科大学药学院,内蒙古 呼和浩特010110) 摇 摇 摘摇 要:目的:从蒙药诃子中提取多糖,并测定其含量。 方法:采用紫外分光光度法,以葡萄糖为对照品,用 -1 苯酚-硫酸显色法测定诃子中多糖的含量。 结果:经方法学研究,多糖在3.212-19.27滋g ·mL 范围内呈线性关 系,回归方程Y=0.0432X +0.0146(r=0.9996,n=6),含量为15.56%,平均回收率为100.4%,RSD=1.60%。 结 论:采用紫外可见分光光度法测定蒙药诃子中多糖含量的方法简便、快速、灵敏,结果准确可靠,适用于诃子中多 糖含量的测定方法。 关键词:蒙药诃子;多糖;苯酚-硫酸法;含量测定 中图分类号:R291.2摇 摇 摇 摇 摇 文献标识码:A摇 摇 摇 摇 摇 文章编号:2095-512X(2014)04-0339-04 EXTRACTION AND CONTENT DETERMINATION OF POLYSACCHARIDE IN MONGOLIAN MEDICINE YU Shu-yan,摇 CHENJian-ping,摇 DU Yan-qing,摇 et al. (College of Pharmacy,InnerMongoliaMedical University,Hohhot010110 China) Abstract:Objective:To explore the extraction and content determination of polysaccharide in the Mongolian medicine Terminalia chebula Retz.Methods:Phenol-sulfuric acid method and UV-PDA spectrophotometer were used to determine content of polysaccharide in Terminalia chebula Retz. With glucose as standard substance.Results:By the methodology study,polysaccharide had a linear relation within the scope of 3.212 to 19.27滋g/ mL,the regression equation was Y=0.0432X +0.0146(r= 0.9996,n = 6),the content was 15.56%,the average recovery was 100.4%,RSD = 1.60%. Conclusion:UV-Vis spectrophotometry is simple,rapid,sensitive,accurate and reliable,which can be used to determine polysaccharide in Mongolian medicine Terminalia chebula Retz. Key words:Mongolian medicine Terminalia chebula Retz. ;polysaccharide;phenol-sulfuric acid method;content determination 摇 摇 诃子(Frctus Terminaliae Chebulae),即本草记载 Retz.)或绒毛诃子(Terminalia chebula Retz.