伴有复杂核型的急性早幼粒细胞白血病的临床及实验研究.pdfVIP

· 552 · M0DERN 0NCOL0GY．Male．2010。VOI．18．NO．03 伴有复杂核型的急性早幼粒细胞 白血病的Ilk床及实验研究 马 力 ，李建勇 ，潘金兰 Clinicalandlaboratoryresearchoftwoacutepromyelocyticleukemiapatientswithcam- plexkaryotype MA J／ ，LIJian—yong，PANJin—lan TheFriendshipHospitalofDalian，Dalian116001，China；TheFirstAffiliatedHospitalofNa ngMedicalUnivemi@，Nanjing210029， China；TheFirstAffiliatedHospitalofSoochowUniversity，Soochow215006，China． A【bstract】Objective：Toexploretheclinicalandlaboratorycharacteristicsofcomplexkaryotypicabnormalitiesof acutepromyelocyticleukemia．M ethods：Multiplexfluorescenceinsituhybridizationwasused incombinationwith conventional cytogeneticsandinterphasefluorescenceinsituhybridizationtostudytwocasesofacutepromyelocytic leukemiapatientswithcomplexkaryotypicabnormalities．Results：Conventionalcytogeneticsdetectedcomplexchro- mosomebanomr alities．Allcomplexchromosomeabnormalitieswereconfirmedpreciselybymultiplexfluorescencein situhybridization．Twopatientshadpoorprognosis．Conclusion：Multiplexfluorescenceinsituhybridizationincom— binationwithconventional cytogeneticsmaycharacterizethecomplexchrmosomalabnormalitiesmoreprecisely．Acute promyelocyticleukemiapatientswithcomplexkaryotypehadabadresponsetochemotherapyandpoorprognosis． K【eywords】leukemia；promyelocytic；acute；complexkaryotypicabnomrality；Multiplexfluorescenceinsituhybrid- ization ModemOncology2010，18(03)：0552—0554 【摘要】 目的：探讨伴有复杂核型的急性早幼粒细胞白血病的临床和实验室特征。方法：应用常规细胞遗传 学，问期荧光原位杂交技术(FTSH)，多重荧光原位杂交技术，检测2例急性早幼粒细胞白血病。结果：常规细 胞遗传学检测发现2例具有复杂核型，问期FISH证实2例均含有PML／RARA融合基因，多重FISH明确复杂 核型性质。2例预后均较差。结论：联合常规细胞遗传学，问期FISH，多重 FISH可以准确明确复杂核型的性 质。具有复杂核型的急性早幼粒细胞白血病患者预后较差。 【关键词】急性早幼粒细胞白血病；复杂核型；多重荧光原位杂交 【中图分类号】R733．7 【文献标识码】A 【文章编号】1672—4992一(2010)03—0552—03 急性早幼粒细胞 白血病 (a