中药对产ESBLs和非产ESBLs菌株的体外抑菌作用.pdfVIP

塞旦匿堂苤查!Q螋生筮!§鲞笙堑塑 4057 ·检验与临床· 陈泽慧 田应彪 叶丽红胡永林 刘清亮 摘要 目的：观察中药对产超广谱8一内酰胺酶(ESBLs)和非产ESBLs茵株的抑茵效果，借此了解中药对 两类细茵的抑茵作用是否存在差异，从而为产ESBLs感染的临床治疗寻求新的途径。方法：使用 Panel MicroScanWalkAway40全自动微生物分析仪及MicroScanNegComboType31复合板进行细菌学鉴定和药敏 试验；将12种中药制备成一定浓度的水煎液。用2倍微孔稀释法测定对产ESBLs和非产ESBLs临床株的最低 其中黄芩、五味子、石榴皮、连翘的抑茵作用最强；其次是黄连、青蒿、血藤、白芍、野菊花。结论：中药对产 ESBLs和非产ESBLs茵株具有一定的抗茵作用。而且敏感性差异无显著性(P0．05)。 关键词 中药；超广谱B一内酰胺酶； 体外抑茵实验 BacteriostaticeffectsoftraditionalChinesemedicineonESBLsandnon．ESBLs strainsCHEN producing Clinical Ze—hui’。TIANYing-biao，YELi—hong，HUYong-lin，LlUQing-liang．卑Departmentof Laboratory．Affiliated Medical HospitalofZunyi 563003，吼ina College，Zunri To thebacteriostaticeffectsoftraditionalChinesemedicineonESBLsand 【AbstractlObjective investigate non．ESBLs strains．Methods automatic andMicroScan producing MicroScanWalkAway40microorganismanalyzer Panel 1wereusedfor identificationand test．Theminimal NegComboType3 bacteriological susceptibility inhibitory and ofdifferentconcentrationsof12kindsoftraditionalChinesemedicineon concentration(MIC)，MIC∞， MIC90 ESBLsandnon．ESBLs strainswer．eevaluated dilutionmethod．ResultsTherewere producing bydouble—mieropore differentlevels effectsonESBLsandnon—ESBLs strainsofthe12kindsof ofbacteriostatic producing medicine。among which，Baical