活血解毒方含药血清对体外培养角质形成细胞cAMP水平的影响.pdfVIP

鱼 n} o靠 l纂铺∽姒丞鞭卷虬 晦黔辫 绢鲻㈣ 骥瓣l耩l翳瓣骚瓣l裙翳l蓬耱瓣鹱薹蕤鬏l辩黼秽端勰拶骥纛蠹焘麓撒熏纛盏l鋈黛霾 活血解毒方含药血清对体外培养角质形成细胞 cAMP水平的影响 刘瓦利1，王君伟1，李敏2，翟瑞洁1，闰雨荷1 [摘要]目的观察活血解毒方含药血清对体外培养角质形成细胞cAMP水平的影响。方法采用清洁级SD 大鼠制备活血解毒方的含药血清，以体外培养角质形成细胞株COLO一16为研究对象，运用酶联免疫检 测法观察不同浓度活血解毒方的含药血清对角质形成细胞内cAMP含量变化的影响。结果不同浓 度动物含药血清与空白对照组相比，差异均具有显著的统计学意义(P均0．05)，细胞中cAMP含量 与动物血清的中药浓度呈正相关。结论活血解毒方药能够提高细胞内cAMP的水平，从而抑制细胞 增殖和促进细胞分化。 [关键词]活血解毒方；含药血清；银屑病；角质形成细胞；cAMP [中图分类号]R285 [文献标识码]A [文章编号] 1001—7089(2010)04—0301—03 EffectofHuoxueDecoction SerumoncAMPConcentrationof inVitro Jiedu Drug-containing Keratinocytes LIU Yu—hel Wa—lil，WANGJun—weil，LIMin2，ZHAIRui-jiel，YAN 7anmen China TraditionalChinese 100053 (1．DepartmentofDermatology，GnangHospitalof Academyof Medicine，Beijing 7anmen Traditional China Chinese China；2．DepartmentofMolecularorganism，GnangHospitalof Academyof Medicine，Bei一 China) 乒增100053 ToobservetheeffectofHuoxuedecoction serumoncAMPconcentration [Abstract]Objective Jiedu drug—containing in invitro．MethodHuoxuedecoction serumwas keratinocytes Jiedu drug—containingpreparedbyusing SDratsofclean afterthe celllineCOLO一16wasculturedin cAMPcon— grade．Then keratinocyte vitro，the centrationin wasdetectedELISAa11．ResultLevelofcAMPin serum keratinocytes by drug—containing andthecontrol had contentincellsand P0．05)difference．cAMP groups groupstatisticallysignificant(all theconcentrationofChinesemedicinein serumare correlated．Conclu