三氧化二砷对胃癌细胞SGC7901多药耐药的逆转作用及其机制.pdfVIP

PharmaceutieaSinica 949 药学学报Acts 2007，42(9)：949—953 三氧化二砷对胃癌细胞SGC7901多药耐药的逆转作用及其机制 薛英威“，韩继广1，李宝馨2，杨宝锋2 摘要：研究三氧化二砷(arsenic t6c,xlde，^e：O，)对胃癌细胞多药耐药的逆转作用及其机制。逐渐递增长春新碱 用；Western VCR细胞对长春新碱(VCR)、5一氟尿嘧啶(5·Fu)及表阿霉素的耐药倍数分别为1656倍、2．69倍及13．05倍。经 05)。 As，0，预处理24h后，长春新碱、5·氟尿嘧啶及袁阿霉素对SGC7901／VCR的耐药倍数显著下降(P0 可能与P—gP、GST．s蛋白表达降低有关。 关键词：胃癌；多药耐药；三氧化二砷 1 中圈分类号：R963：R979文献标识码：A 文章编号：0513—4870(2007)09—0949—05 ReversalandmechanismofarsenictrioxideOR effect multidrug of resistance carcinomacellsSGC7901 gastric XUE Bao．xin2，YANG Ying．wei“，HANJi．guan91，LI Bao．fen92 Abdominal Medical (J HospitalofHarbin 150040，China Surgery，Third University，Harbin Medical oyPharmacy．HarbinUniversity，Harbin150086，China) 2．College ofthis isto thereversaletieetanditsmechanismofarsenic Abstract：The purpose study investigate resistanceof carcinomacells．Theconcentrationofvincristine trioxide(As203)011multidrug gastric inducethe of cell (VCR、increasedto resistance carcinoma gradually drug gastric wasusedtodeterminethelethaleffectof OiltumorcellsandWestern was anticarcinogens blottingassay todeterminethe of applied expressionP—glucoprotein(P—gP)andglutathione tumorcells．Asa resistanceofSGC7901／VCRcellst