大黄的研究.docVIP

大黄对急性胰腺炎T淋巴细胞亚群的改变的影响 姜良富 [摘要] 目的 探讨重症急性胰腺(SPA)T淋巴细胞亚群的改变及大黄的治疗作用，并对其作用机制进行探讨。方法 健康Wistar大鼠20只随机分成二组：SAP模 型组(M组，n=10)，大黄治疗组(T组，n=10)，以5%牛磺胆酸钠溶液胰胆管逆行推注复制大鼠SAP模型；治疗组在复制模型后即刻胃管内注入生大黄粉溶液:流式细胞仪 检测AP大鼠外周血中T淋巴细胞亚群CD4+/CD8+以及CD4+/CD8+的变化；结果 M组血中CD4+阳性细胞数以及CD4+/CD8+值明显低于T组(P0.05)，与M组比较，T组胰腺 病理变化明显减轻；结论 1)SAP时存在细胞免疫功能抑制，2)大黄能增强机体的细胞免疫功能，对SAP有一定治疗作用。 [关键词] 急性胰腺炎；T淋巴细胞亚群；大黄 [Abstract] Objective To explore the changes in the lymphocyte subpopulations in peripheral blood in experim-ental severe acute pancreatitis in rats and the effect of Rhubarb on them. Methods Twenty Wistar rats were randomly divided into two groups: SAP Model group (M, n=10), Therapeutic group (T, n=10), SAP group were induced by injecting adversely 5% sodium taurocholate into the common biliary-pancreatic duct; Therapeutic group were made as same as M group besides Rhubarb were injected into their stomach on the instant after operation; Peripheral blood samples were drawn after 24hours of models making, the percentages of CD4+ and CD8+ lymphocyte were determined by the fl ow cytometry. Results the percentages of CD4+ and CD4+/CD8+ratio in SAP were signifi cantly lower than those T group. The pathological changes of pancreas in T group are lighter than M group. Conclusion Damage to cellular immunity function occurs in SAP. Rhubarb has therapeutic effi cacy on SAP,the potential mechanisms may be the improvement of cellular immune function. [Key words] Severe Acute Pancreatitis; T lymphocyte subset Rhubarb; Rat 肝胆汁流量，而且能促进排胆，松驰奥狄括约肌，再结合大黄广 谱的抗菌、消炎、抗毒作用，可用于治疗胆系感染、胆石症等。 沈氏等Ⅲ 用大黄治疗大肠杆菌感染大鼠造成急性重症胆管炎 模型的实验研究表明，大黄合剂能明显降低动物的自由基、磷 酸酶A：浓度，纠正PGFlot(前列环素的代谢产物)／TXB：(血栓 素B：)比值的失衡，且大黄合剂有抑菌减毒作用，使细胞因子 及炎症介质生成的连锁反应受到一定程度的抑制，可能为其治 疗胆管炎的机制之一。黄氏等 研究表明，大黄能明显降低 幼鼠血清TBil、DBil、ALT、ALP及TBA水平，显著降低胆汁酸毒 性作用，减轻肝损伤；大黄治疗肝内胆汁淤积作用机制可能与 降低肝组织NO水平，减轻脂质过氧化反应、增强细胞保护作 用和提高抗氧化损伤能力有关。刘氏等 研究表明，大黄组 大鼠胃肠肌电活动(MMC)节律恢复时间较模型组早，周期无 明显改变，胃肠NOS的表达也比模型组显著减少(P0．05)。 大黄通过增加胆汁排泌，促进MMC的恢复，从而达到清热解 毒、利胆退黄的效应。邱氏等 。。研究表明大黄煎剂组大鼠血 清ALT、AST、NH，、LPS水平显著降低，肠内容物PH值下降，肝 线粒体对