定量光导荧光技术评价含氟牙膏抑制早期龋损的效果_医学论文.docVIP

定量光导荧光技术评价含氟牙膏抑制早期龋损的效果_医学论文 定量光导荧光技术评价含氟牙膏抑制早期龋损的效果_医学论文 【摘要】 目的 通过在体外形成牙釉质光滑面早期龋损的恒化器龋病模型,评价定量光导荧光技术反映光滑面早期龋损矿物质含量变化的效果。 方法 将30个牙釉质块开窗后分为3组,分别使用氟含量1 450 mg/L的氟化钠牙膏、单氟磷酸钠牙膏和无氟牙膏的处理液处理,并在体外恒化器龋病模型中进行脱矿循环, 定量光导荧光技术(QLF)分析病损脱矿后的面积、荧光损失和总荧光损失量,激光扫描共聚焦显微镜(CLSM)分析脱矿后矿物质含量变化。 结果 在体外恒化器龋病模型中,无氟组形成的病损面积较大,荧光损失量较高,总荧光损失量以10为底的对数值较大(0.05),氟化钠组和单氟磷酸钠组的差别无统计学意义(Pgt0.05)激光共聚焦显微镜观察显示,脱矿循环中无氟组形成的病损在荧光面积、平均荧光量和总荧光量3个参数上都明显高于其他两组,差别有统计学意义(0.05)。QLF分析与CLSM结果具有较好的相关性(rgt0.70)。 结论 2种氟浓度1 450 mg/L的牙膏与无氟牙膏比较,具有明显的抑制牙釉质光滑面早期龋损脱矿的作用。QLF分析结果能够客观的反映脱矿病损区矿物质含量的变化。 【关键词】 氟 牙膏 龋齿 牙釉质 荧光 显微镜检查,共焦 ABSTRACT: Objective The objective of the study is to assess the effect of quantitative light-induced fluorescence(QLF) in monitoring demineralization of early caries on smooth surfaces by comparing it with confocal laser scanning microscope through bacteria models in vitro. Methods Enamel surface specimens were randomly allocated into 3 groups: 1 450 mg/L sodium fluoride dentifrice group, 1 450 mg/L sodium monofluorophosphate dentifrice group and non-fluoride dentifrice control group. Sound enamel surfaces were dematerialized in bacteria model in vitro. QLF was used to analyze the area of lesions(Area), the loss of fluorescence(ΔF) and ΔQ(Area×ΔF) on enamel surfaces after demineralization. Confocal laser scanning microscopy(CLSM) was used to quantify the mineral content after demineralization. Results Area, ΔF and ΔQ in non-fluoride dentifrice group were significantly higher than other two fluoride groups after demineralization(0.05). No statistical difference was found between two fluoride groups(Pgt0.05). Results from CLSM supported that area of the fluorescent lesion, total fluorescence and average fluorescent of the lesion in non-fluoride dentifrice group were higher than other two groups after demineralization(0.05). QLF and CLSM showed fine correlation after demineralization(rgt0.70). Conclusion Sodium fluoride and sodium monofluorophosphate dentifrice(F 1.450 mg/L) in reversal of early caries w