宫内缺氧对新生大鼠神经元的影响及当归的保护作用_药学论文.docVIP

宫内缺氧对新生大鼠神经元的影响及当归的保护作用_药学论文 宫内缺氧对新生大鼠神经元的影响及当归的保护作用_药学论文 作者：岳鹤声 陈旭东 刘红敏 华新宇 余鸿 【摘要】 目的探讨宫内缺氧对新生大鼠海马神经元的影响及当归注射液的保护作用。方法孕期14 d的SD大鼠21只，随机分为对照组、缺氧组和当归治疗组。缺氧组孕鼠自孕第14天开始经尾静脉注射生理盐水8 ml/kg，1次/d，连续7 d，后用低张性缺氧模型致胚鼠宫内缺氧2 h，连续3 d(孕期第14，15，16天);当归治疗组用250 g/L的当归注射液代替生理盐水，其余处理同缺氧组;对照组不缺氧，其余同缺氧组。孕鼠分娩后立即取新生鼠脑组织，常规石蜡切片，经神经元特异性烯醇化酶(Neuron specific enolase，NSE)免疫组织化学染色后行图像分析。结果缺氧组新生鼠海马NSE免疫组化阳性细胞的积分光密度(Integrated Optical Density，IOD)值较对照组减少〔IOD缺氧组：(4.9±1.1);IOD对照组：(6.2±1.6),0.05〕;当归组新生鼠海马NSE免疫组化阳性细胞的IOD值较缺氧组增加〔IOD治疗组：(6.9±2.6), 0.05〕。结论一定程度的缺氧可刺激海马CA3区神经元变性;当归注射液对宫内缺氧新生大鼠海马CA3区神经元可能具有保护作用。 【关键词】 当归; 缺氧; 神经元; 免疫组化 　　Abstract：ObjectiveTo study the effect of intrauterin hypoxia on neuron of hippocampus from newborn rats and the protective role of Angelica injection.Methods14d-conceiving female SD rats(n=21) were divided randomly into three groups:control group(CG)、hypoxia group(HG) and Angelica group(AG).Since the fourteenth days after they were pregnant the rats of HG were injected with normal Saline through caudal veins (within 7 straight days ; 8ml/(kg.d)before intrauterin hypotonic hypoxia (within 3 straight days;on the fourteenth、fifteenth and sixteenth day after pregnancy;2 hours everyday ).Angelica sinensis injection instead of normal saline was injected into the rats in the AG ,and other procedures were the same as the HG.No administration of hypoxia was performed in the CG.Take out their brain tissues immediately of newborn rats after childbirth.The expression of NSE in the hippocampus of newborn rats was studied with immunohistochemistry and the results were analyzed by image processing system.ResultsIOD of NSE positive cells in HG was lower than that of the CG〔IODHG：(4.9±1.1);IODCG：(6.2±1.6), 0.05〕;But in AG,them are higher than that of HG〔IODAG：(6.9±2.6),0.05〕.ConclusionHypoxia can stimulate newborn rats neuron of CA3 of hippocampus into degeneration ,Angelica injection can play a protective role to neuron of CA3 of hippocampus from newborn rats with intrauterin hypoxia.