微波辅助提取土茯苓多糖_医学论文.docVIP

微波辅助提取土茯苓多糖_医学论文 微波辅助提取土茯苓多糖_医学论文 作者：黄少伟 池汝安 张越非【摘要】 目的筛选土茯苓多糖提取的最优化工艺。方法应用微波辅助提取技术，通过单因素和正交实验，以土茯苓多糖提取率为指标，筛选土茯苓多糖的最佳提取工艺条件。结果土茯苓多糖最佳提取工艺条件为：微波功率800W，固液比（g/ml）1∶30,提取温度100℃，回流浸提两次，10 min/次，浓缩液加90％乙醇醇析。结论优化后的工艺稳定，微波辅助提取土茯苓多糖，提取时间短、提取率高，另有较大部分土茯苓总黄酮也同时被浸出。按照优化后工艺条件实验，土茯苓多糖提取率、粗多糖得率以及粗多糖中多糖含量分别为8.33％，3.97％和39.5％。 【关键词】 微波辅助提取 土茯苓 多糖 总黄酮 　　Abstract:ObjectiveTo optimize the process of extracting polysaccharides from Smilax glabra Roxb. MethodsWith the extraction rate of polysaccharides as an examinable target, the optimal extraction conditions of polysaccharides has been proposed by microwave-assisted method through single factor experiment and orthogonal test method. ResultsThe optimal technological parameters of extracting polysaccharides were as follows: the microwave power, the solid-liquid ratio, the extract temperature and extract time were 800 W, 1∶30（g/ml）, 100℃ and 10min twice, respectively. Lastly, the crude polysaccharides were obtained by adding ethyl alcohol into the treated leachate after laying a period.ConclusionThe proposed method is stable and reliable for the extraction of polysaccharides from Smilax glabra Roxb., which has the advantages of short extraction time, high extraction rate and obtaining general flavonoids simultaneously with polysaccharides. Under the optimized operation conditions, the extraction ratio, extraction yield of crude polysaccharides and the content of polysaccharides in the crude polysaccharides were 8.33%, 3.97% and 39.5%, respectively. 　　Key words:Microwave-assisted extraction； Smilax glabra Roxb.； Polysaccharides； General flavonoids 土茯苓为百合植物光叶菝葜Smilax glabra Roxb.的干燥根，又名红土苓，其味甘、淡、平，归肝、胃经，具有除湿，解毒，通利关节作用，用于湿热淋浊、带下病、痈肿、瘰疬、疥癣、梅毒及汞中毒所致肢体拘挛,筋骨疼痛，为常用中药材［1］。土茯苓主要化学成分为黄酮、皂苷等，实验中发现较多的多糖类成分也存在于土茯苓中。鉴于多糖具有增强机体免疫力及抗癌等多方面生理活性，本实验对土茯苓中多糖的提取工艺进行了研究。 微波辅助提取技术是近年来发展起来的一种新方法，具有选择性好、快速、高效、安全、节能等优点。本文首次报道了微波辅助提取技术在土茯苓多糖提取中的应用，通过单因素和正交实验，采用水提醇沉法研究土茯苓多糖的优化提取工艺，以期对土茯苓药材的应用提供基本资料。 　　1 材料与方法 　　1.1 原料及试剂 　　土茯苓饮片购于三九中药房，经湖北中药研究院王克勤研究员鉴定为百合植物光叶菝葜Smi