心钠素对兔油酸型急性肺损伤血及支气管肺泡灌洗液中TNF┐α，IL┐8，ET┐1含量的影响_医学论文.docVIP

心钠素对兔油酸型急性肺损伤血及支气管肺泡灌洗液中TNF┐α，IL┐8，ET┐1含量的影响_医学论文 心钠素对兔油酸型急性肺损伤血及支气管肺泡灌洗液中TNF┐α，IL┐8，ET┐1含量的影响_医学论文 作者：林允信　张丙芳　戚好文　李志超　贾斌 【关键词】 急性肺损伤 　　关键词: 急性肺损伤心钠素肿瘤坏死因子白细胞介素-8内皮素-1支气管肺泡灌洗 　　摘 要:目的 探讨心钠素（ANP）对油酸型急性肺损伤（ALI）家兔血及支气管肺泡灌洗液（BALF）中TNF-α，IL-8和ET-1含量的影响. 方法 观察油酸型ALI家兔（n=6）ANP治疗前，后不同时间混合静脉血及BALF中上述3种因子含量的变化，并于实验结束后测定肺水质量分数. 结果 油酸致伤后30，60和120min血及BALF中TNF-α，IL-8和ET-1含量均显著增高（0.05～0.01），ANP治疗后各因子含量明显下降（0.05），并维持在低水平，肺水质量分数也显著降低（0.05）. 结论 iv ANP可有效降低油酸致伤后血及BALF中TNF-α，IL-8，ET-1水平，抑制肺内炎症反应，减轻肺损伤. 　　Keywords:acute lung injuryatrial natriuretic peptidetu-mour necrosis factorinterleukin-8endothelin-1bronchoalveolar lavage 　　Abstract:AIM To investigate the effects of atrial natriuret-ic peptide（ANP）on the levels of TNF-α，IL-8and ET-1in blood and bronchio-alveolar lavage fluid（BALF）from a rab-bit acute lung injury（ALI）model induced by oleic acid.METHODS The concentrations of the three cytokines in mixed venous blood and BALF were continuously analysed at different time points before and after being treated with ANP（0，30，60，120min）.Additionally，extravascular lung water was observed at the end of the study.RESULTS The con-centrations of the three cytokines in blood and BALF were markedly increased at30，60and120min after intravenous infusion of oleic acid（0.05～0.01）.In contrast，the lev-els of the three cytokines remarkably decreased and kept at a lower state after the intravenous infusion of ANP（0.05）.So did the extravascular lung water（0.05）.CONCLUSION The intravenous infusion of ANP may effi-ciently decrease the levels of TNF-α，IL-8and ET-1in blood and BALF after infusion of oleic acid，inhibit intrapulmonary inflammation and reduce the lung injury. 　　0 引言 　　急性肺损伤（acute lung injury，ALI）的特征是以肺泡-毛细血管膜损伤、通透性增高为主要病理生理改变，并可发展为急性呼吸窘迫综合征（acute res-piratory distress syndrome，ARDS）.ALI目前尚无满意的药物治疗方法，病死率高达50%～70%.近年随着对全身炎症反应机制认识的深入，明确了肺内炎症反应在ALI发病中的重要作用.抑制和阻断ALI时肺内炎症反应，改善肺泡-毛细血管膜通透性是当前ALI治疗的主要着眼点之一.新近资料显示心钠素（ANP）对ALI具有保护作用，可降低ALI动物肺动脉高压，减轻其肺内液体渗出［1-3］ ，但其抑制肺内炎症反应的作用机制，国内外报道较少.本研究拟观