桂枝甘草汤方药半仿生提取等4种方法提取液的成分比较_药学论文.docVIP

桂枝甘草汤方药半仿生提取等4种方法提取液的成分比较_药学论文 桂枝甘草汤方药半仿生提取等4种方法提取液的成分比较_药学论文 【摘要】 　　目的优选桂枝甘草汤方药成分的提取工艺。方法以甘草次酸、肉桂酸、总黄酮、挥发油、干浸膏的含量为指标,在药材粒度、溶剂量、煎提温度、滤过、浓缩等条件相同的前提下,采用半仿生提取法(SBE法)、水提取法(WE法)、半仿生提取醇沉法(SBAE法)、水提取醇沉法(WAE法)进行比较研究。结果5个指标综合评价Y值为:SBE法＞WE法＞SBAE法＞WAE法。结论 桂枝甘草汤方药成分的提取以用SBE法为佳。即3次煎提用水的pH值依次为：2.00，7.20，8.50，煎煮的时间依次为190，90，50 min。 【关键词】 桂枝甘草汤 半仿生提取法 综合指标 　　Abstract：ObjectiveThe extraction technologies for the components of Guizhi Gancao Decoction were optimized.MethodsFour methods: the semi -bionicextraction(SBE), the water extraction(WE), the semi-bionicextraction by precipitation with alcohol(SBAE) and the water extraction by precipitation with alcohol(WAE) were used to extract Guizhi Gancao Decoction,with glycyrrhetinic acid，cinnamic acid，total flavones，volatile oil and dry extract taken as the marker,under the same conditions of druggranularity, solventamount, decocting temperature, filtration, concentration, etc., and then four methods were compared and studied.Results Through the comprehensive evaluation of seven markers, their comprehensive values Y decreased in the order of SBE, WE, SBAE and WAE. ConclusionSBE is better than the other three methods in the extraction of the components of Guizhi Gancao Decoction, which is shown by the fact that the pH values of the water for the 1st, 2nd and 3rd decoctions are 2.00，7.20，8.50 and the extraction times are 190，90 min and 50 min respectively. 　　Key words： Guizhi Gancao Decoction； SBE method； Comprehensive index 　　桂枝甘草汤由桂枝、炙甘草组成。具有补心助阳、升阳化气、补阳扶中的作用。根据SBE法理论［1～6］，在用均匀设计优选出桂枝甘草汤方药SBE法工艺条件和醇沉较佳浓度的基础上［7，8］，为进一步探讨该方药用SBE法提取是否较目前普遍适用的提取方法为佳,故以甘草次酸、肉桂酸、总黄酮、挥发油、干浸膏得率为指标,对该方药做了4种提取方法的平行对照实验。 　　1 仪器与药品 　　pHS3C型精密pH计(上海雷磁仪器厂)；760双光束紫外可见分光光度计（上海精密科学仪器有限公司）；MA110型电子分析天平(上海第二分析仪器厂)；LXJ型离心沉淀机(上海第三分析仪器厂) ；754紫外可见分光光度计（上海精密科学仪器有限公司）；vp高效液相色谱仪 (SHIMADZU CORPORATION ASSEMBLED IN CHINA)，SPD检测器，LC泵； KQ型医用超声波清洗器（昆山市超声仪器有限公司）；RE型旋转蒸发器（上海亚荣生化仪器厂）。 　　药材经张兆旺鉴定，桂枝为樟科植物肉桂Cinnamomum cassia Presl的干燥嫩枝，甘草为豆科植物甘草GLycyrrhiza uraLensis Fisch的干燥根及根茎，炙甘草：取