毛泡桐叶中乌索酸的提取分离及反相高效液相色谱分析_药学论文.docVIP

毛泡桐叶中乌索酸的提取分离及反相高效液相色谱分析_药学论文 毛泡桐叶中乌索酸的提取分离及反相高效液相色谱分析_药学论文 作者：易艳萍，邹盛勤，谢晚彬，陈武 【摘要】 　　目的提取鉴定毛泡桐叶中的乌索酸，并建立其含量测定方法。方法采用“醇提凝析法”新工艺分离制备乌索酸，IR，MS，NMR 鉴定其结构。建立反相高效液相色谱测定其含量的方法，色谱柱为 KromasilC18 柱，流动相为乙腈-甲醇-水-磷酸（体积比168∶682∶150∶1.68），流速0.8 ml/min，检测波长210 nm，柱温25℃。结果乌索酸在0.92～16.56 μg范围内线性关系良好（r=0.999 6）。样品乌索酸含量为99.61%，得率为30.5%。结论 “醇提凝析法”分离制备乌索酸工艺先进，实用可行，为工业试验生产乌索酸提供了技术条件；RP-HPLC法测定乌索酸含量准确、精密度高、重复性好、线性范围宽，可作为控制乌索酸质量的方法。 【关键词】 毛泡桐叶 乌索酸 结构表征 反相高效液相色谱 　　Abstract：Objective：To extract and identify ursolic acid from Paulownia tomentosa (Thunb. ) Steud leaves and to establish a determination method of ursolic acid. Methods：Ursolic acid was extracted from Paulownia tomentosa(Thunb. ) Steud leaves by adopting a new method of ethanol extraction and agglutination separation, and its structure was identified by IR, MS and NMR. A determination method of ursolic acid in Paulownia tomentosa (Thunb.) Steud leaves by reversed- phase high performance liquid chromtograph was established. The chromatographic column (KromasilC18, 4.6 mm×250 mm, 5 μm), acetonitrile-methanol-water-phosphoric acid mobile phase(168:682:150:1.68, V/V) with 0.8 ml/min flow rate, the detected wavelength(210 nm) and the column temperature (25℃) were adopted. Results：Ursolic acid showed good linear relationship in the range of 0.92～16.56 μg(r=0.999 6). The average recovery rate was 99.82% (RSD was 0.75%). The content was 99.61% and the gain rate was 3.05 ‰. Conclusion：The method of ethanol extraction and agglutination separation to extract ursolic acid is advanced, rational, practical and feasible, and it provides technology condition for industrial production of ursolic acid. RP-HPLC is simple and accurate, it has good repeatability and wider linear range, and it can be used to evaluate the quality of ursolic acid. 　　Key words：Paulownia tomentosa (Thunb.) Steud leaf; Ursolic acid; Structural characteristics; RP-HPLC 　　毛泡桐叶为玄参科泡桐属植物毛泡桐Paulownia tomentosa (Thunb.) Steud 的叶子［1］。泡桐的药用价值我国古代就有记载，《本草纲目》（1578年）记有“桐叶主恶蚀疮著阴皮主五痔、杀三