灯盏花总黄酮与微量元素含量分析及其药效机理的研究_药学论文.docVIP

灯盏花总黄酮与微量元素含量分析及其药效机理的研究_药学论文 灯盏花总黄酮与微量元素含量分析及其药效机理的研究_药学论文 作者：董顺福 韩丽琴 刘建华 赵文秀 董宏博 【摘要】 　　目的研究中药灯盏花中总黄酮及微量元素的含量，为治疗心脑血管疾病的机理提供科学的依据。方法采用醇提法提取灯盏花中总黄酮，紫外分光光度法测定药物中总黄酮的含量，测定吸收波长为λ=510 nm；采用HNO3-HClO4湿法消化，原子吸收分光光度法测定钾、钙、钠、镁、铁、铜、锌、锰、镍9种微量元素的含量。结果测定结果显示，灯盏花中含有丰富的微量元素和黄酮类化合物，微量元素含量较高的是铁、铜、锰元素。结论 实验结果为研究中药灯盏花中黄酮类化合物与微量元素在治疗心脑血管疾病中的作用机制，为临床合理应用和开发中药灯盏花提供了有用的数据。 【关键词】 灯盏花 总黄酮 微量元素 　　Abstract:ObjectiveClinical studies indicated that the flavone and trace elements play a beneficial role to maintaining the cardio-cerebral vascular system functions. The contents of flavone and trace elements in Erigeron Breviscapus（Vant）Hand.-Mazz were determined to study the basis of the effect that the flavone and trace elements in Chinese herbal medicines with cardio-cerebral vascular diseases . MethodsThe flavone in Erigeron Breviscapus（Vant）Hand.-Mazz was extracted by alcohol and the content of flavone was determined at the wavelength of 510nm by ultraviolet spectrophotometry. The contents of nine trace elements and constant elements in Erigeron Breviscapus（Vant）Hand.-Mazz, including potassium, calcium,sodium, magnesium,iron,copper,zinc, manganese and nickel were determined by flame atomic absorption spectrophotometry, the sample was treated with HNO3-HClO4.ResultsThe results showed that there were comparatively rich trace elements and flavone, among which are comparatively high iron,copper and manganese in Erigeron Breviscapus（Vant）Hand.-Mazz . ConclusionIt provided useful data for discussing the basis of effect the content of flavone and the trace elements cure for cardio-cerebral vascular diseases in Erigeron Breviscapus（Vant）Hand.-Mazz and for using Erigeron Breviscapus（Vant）Hand.-Mazz in clinical studies. 　　Key words:Erigeron Breviscapus（Vant）Hand.-Mazz； Flavone； Trace element 　　灯盏花又名灯盏细辛，是菊科植物短葶飞蓬［Erigeron breviscapus（Vant）Hand.-Mazz］的植物，含有黄酮、吡喃酮、倍半萜等五十多种化学成分［1］，具有活血舒筋、止痛消积等功效，临床上广泛用于治疗脑梗塞、缺血性心脏病等心脑血管疾病，其有效成分之一为总黄酮［2］。现代研究表明，微量元素与人体的生物功能具有密切的关系［3］，为研究中药灯盏花中微量元素和总黄酮的含量与治疗心脑血管疾病的关系，本实