灯盏花素对大鼠脑缺血再灌注后脑内Fas表达的抑制作用_药学论文.docVIP

灯盏花素对大鼠脑缺血再灌注后脑内Fas表达的抑制作用_药学论文 灯盏花素对大鼠脑缺血再灌注后脑内Fas表达的抑制作用_药学论文 【摘要】 目的 研究灯盏花素对大鼠脑缺血再灌注引起脑损伤的保护作用，探讨其作用机制。方法 选用40只雄性Wistar大鼠，随机分成5组：假手术组、生理盐水组（IR组）、MgSO4治疗组、灯盏花素治疗组Ⅰ和Ⅱ（BreⅠ、Ⅱ组）。结扎中脑动脉，然后再灌注。用TUNEL法和免疫组化法分别检测脑组织凋亡细胞和Fas阳性细胞的变化。结果 ①降低脑组织凋亡细胞：MgSO4组、BreⅠ、Ⅱ组在脑缺血再灌注23 h 后海马区凋亡神经元/海马神经元均较IR组海马区凋亡神经元/海马神经元低，差异有显著性。②降低Fas阳性表达细胞数量：MgSO4组、BreⅠ组和BreⅡ组在脑缺血再灌注23 h 后海马区Fas表达阳性神经元/海马神经元均较IR组海马区Fas表达阳性神经元/海马神经元低，差异有显著性。结论 灯盏花素可显著保护大脑缺血再灌注引起的脑损伤,可能与其抑制Fas蛋白的表达有关。其作用优于单用MgSO4 。 【关键词】 灯盏花素；脑缺血；再灌注；凋亡细胞；Fas蛋白 　　Abstract:Objective To study the protective effect and mechanism of breviscapine on rat cerebral damage after ischemiareperfusion. Methods 40 male Wistar rats were divided into five groups: sham group, IR group, MgSO4 treatment group, breviscapine treatment group Ⅰ and Ⅱ. The brain artery was ligated and then infused. Apoptosis of brain cells and changes of Fas positive cells was identified by TUNEL method and immunohistochemical method respectively. Results ① Apoptosis of neurons cells in brain was reduced: Compared with IR group, MgSO4 group, Bre Ⅰ group and Bre Ⅱ group showed lower hippocampus apoptosis neurons / hippocampal neurons 23 hrs after ischemiareperfusion, and Bre group was lower in apoptosis neurons than MgSO4 group, the difference was significant. ② The number of Fas positive cells in cerebral was reduced: Compared with IR group, MgSO4 group, Bre Ⅰ group and Bre Ⅱ group were lower in hippocampus Faspositive neurons / hippocampal neurons 23 hrs after ischemiareperfusion, and Bre group was lower than MgSO4 group in apoptosis, the difference was significant. Conclusion Breviscapine could significantly protect the brain from ischemia reperfusion brain injury, what might be related to inhibition of Fas protein expression. Its effect was better than MgSO4. 　　Key words:breviscapine; cerebral ischemia; reperfusion;apoptosis cells;Fas protein 　　脑血管缺血性疾病是高致残率和高死亡率的疾病，神经保护药物将有助于减轻缺血半暗区的损伤，促进脑功能的恢复。灯盏花素(breviscapine，Bre)是从中药灯盏花中提取的，其有效成份是4’羟基黄芩素7O葡萄糖醛酸苷［1］，具有显著的