瑞香狼毒提取物和肿瘤坏死因子对肺癌基因蛋白表达的影响_药学论文.docVIP

瑞香狼毒提取物和肿瘤坏死因子对肺癌基因蛋白表达的影响_药学论文 瑞香狼毒提取物和肿瘤坏死因子对肺癌基因蛋白表达的影响_药学论文 【摘要】 目的探讨瑞香狼毒和肿瘤坏死因子(TNF)对肺癌细胞基因蛋白表达的影响。方法用瑞香狼毒水提取物和TNF分别及联合处理肺癌细胞株NCI-H157和NCI-H446，用免疫组织化学SP法分别检测用药前后肺癌细胞中多药耐药相关蛋白(MDM2)、肺耐药蛋白(LRP) 和热休克蛋白27(HSP27)的表达状况。通过形态学图像分析，比较瑞香狼毒和TNF用药前后三种基因蛋白表达的差异。结果用药前后两种肺癌细胞株3种基因蛋白表达情况如下：①瑞香狼毒组NCI-H446细胞株用药后LRP和MDM2阳性细胞灰度值均高于用药前(0.05)；HSP27的表达用药前后差异无显著性(Pgt;0.05)。NCI-H157细胞株用药后LRP和HSP27阳性细胞灰度值均高于用药前 (0.05)；MDM2的表达用药前后差异无显著性(Pgt;0.05)。②TNF组NCI-H446细胞株用药后LRP阳性细胞灰度值高于用药前 (0.05)；HSP27和MDM2的表达用药前后差异均无显著性(Pgt;0.05)。NCI-H157细胞株用药后LRP，MDM2和HSP27阳性细胞灰度值均高于用药前(0.05),其中LRP具有高度显著性(0.01)。③联合用药组NCI-H446细胞株用药后LRP和MDM2阳性细胞灰度值均高于用药前(0.05),HSP27的表达用药前后差异无显著性(Pgt;0.05)。NCI-H157细胞株用药后LRP、MDM2和HSP27阳性细胞灰度值均高于用药前 (0.05),且LRP和HSP27差异具有高度显著性(0.01)。结论瑞香狼毒提取物和肿瘤坏死因子对两种肺癌细胞株的某些基因蛋白的表达具有抑制作用；两种药物联合应用对基因蛋白的表达有明显的抑制作用。 【关键词】 瑞香狼毒 肿瘤坏死因子 肺癌细胞株 基因蛋白 　　The Effects of Extracts from Ruixianglangdu and Tumour Necrosis Factor of Expression of Gene Protein in Two Lung Carcinoma Cell 　　Abstract:ObjectiveTo research the effect of extracts from Ruixianglangdu and tumour necrosis factor(TNF) on expression of gene protein .MethodsTwo lung carcinoma cell strains were treated with extracts from Ruixianglangdu and TNF,the expressions of MDM2,LRP and HSP27 in lung cancer cell strains were detected with immunohistochemistry SP assay before and after being treated respectively. To compare the difference of expression of three kinds of gene protein with morphological image assay.ResultsThe difference of expressive intensity of MDM2,LRP and HSP27 between before and after being treated with extracts from Ruixianglangdu and TNF in two lung carcinoma cell strains as follows. ①In Ruixianglangdu group:in lung carcinoma cell strain NCI-H446 the expressive intensity of LRP and MDM2 were higher than that before treated(0.05),except HSP 27(Pgt;0.05).②In NCI-H157 the expressive intensity of LRP and HSP 27 were higher than that after treated(0.05)，except MDM2 (Pgt;0.05). ③In TNF group: the expressive intensity of LRP was higher than