砂仁提取液对离体家兔主动脉条收缩性能的影响_药学论文.docVIP

砂仁提取液对离体家兔主动脉条收缩性能的影响_药学论文 砂仁提取液对离体家兔主动脉条收缩性能的影响_药学论文 作者：冯广卫， 陶玲， 沈祥春， 郝明， 彭佼 【关键词】 砂仁；,,去甲肾上腺素；,,氯化钾；,,胸主动脉 　　摘要：目的研究砂仁提取液对兔离体胸主动脉血管条的作用。方法采用家兔离体胸主动脉血管条，以不同浓度去甲肾上腺素(NE)、氯化钾(KCl)为刺激剂，观察砂仁提取液对其量效曲线的影响；以NE为血管收缩刺激剂，观察砂仁提取液对内钙释放与外钙内流的影响。结果砂仁提取液使NE与KCl的量效曲线明显右移，最大反应性降低，对KCl量效曲线的抑制作用强于NE；在无钙Kerbs液中，砂仁提取液浓度依赖性抑制NE诱导的内钙释放所诱导的血管收缩反应，对复钙后NE所致的血管持续收缩具有抑制作用的趋势。结论 砂仁提取液可舒张兔主动脉条，该作用可能与钙离子拮抗有关，且对主动脉依内钙性收缩的敏感性大于依外钙性收缩；对电压依赖钙通道的拮抗作用强于受体操纵的钙通道。 　　关键词：砂仁； 去甲肾上腺素； 氯化钾； 胸主动脉 　　Effects of Amomum Villosum Extraction on the Contraction of Rabbit Thoracic Aorta in vitro 　　　Abstract：ObjectiveTo investigate effects of Amomum villosum extraction (AVE) on contraction of rabbit thoracic aorta in vitro.MethodsThe isotonic contraction of the thoracic aorta strips in rabbits was recorded, and the effects of AVE on the concentrationresponse curves of noradrenalin, high potassium was observed. The procedure of freeCa2+ kerbs and Ca2+ addition were designed to indirectly observe the effects of AVE on intracellular free Ca2+ stimulated by NE.ResultsAVE shifted the concentration-response curves of NE and KCl, and the maximum responses decreased. In Ca2+- free medium, AVE concentration-dependently inhibited the transient induced by NE and it was tended to inhibit the longlasting contraction induced by addition of Ca2+.ConclusionAVE has significant relaxing effect on the contraction of thoracic aorta strips. The effect may be due to the inhibition of the calcium channel of aortic smooth muscle. AVE shows relatively less effect on the extracellular Ca2+dependent. than that on the intracellular Ca2+dependent contraction, and more against effects on potential dependent Ca2+ channel than receptors operated channel. 　　Key words：Amomum Villosum Extraction； Noradrenalin； KCl； Thoracic aorta 　　中药砂仁为姜科植物阳春砂 Amomum villosum Lour.，绿壳砂 Amomum villosun Lour.var. xanthioides T.L. Wu et Senjen 或海南砂 Amomum longiligulare T.L.Wu的干燥果实，目前《中国药典》收载的砂仁共有3种，具有化湿开胃，温脾止泻，理气安胎的功效［1］；同时砂仁也是我国民间常用的重要香料物质。国内外对砂仁进行了大量的研究，但砂仁化学及药理活性成