砂生槐水提取物对免疫抑制型小鼠免疫功能的影响_药学论文.docVIP

砂生槐水提取物对免疫抑制型小鼠免疫功能的影响_药学论文 砂生槐水提取物对免疫抑制型小鼠免疫功能的影响_药学论文 作者：安方玉，刘英，马兴铭，田卫花，郭君，雒燕萍 【摘要】 　　目的研究砂生槐水提取物对免疫抑制型小鼠免疫功能的影响。方法通过给小鼠腹腔注射环磷酰胺，建立免疫功能抑制模型，并灌服砂生槐水提取物［0.6～2.4 g/(kg·d)］。以胸脾指数，B淋巴细胞的增殖，抗绵羊红细胞特异抗体的生成，腹腔巨噬细胞的吞噬作用为测定指标。结果与模型对照组比较，砂生槐水提取物3个剂量组均可提高免疫抑制小鼠脾脏指数（0.01），可协同LPS促进免疫抑制小鼠B淋巴细胞的增殖（0.01），高、中剂量组可促进免疫抑制模型小鼠抗绵羊红细胞特异抗体的生成（0.01，0.05），3个剂量组均能增强免疫抑制小鼠巨噬细胞的吞噬功能（0.05，0.01，0.01）。结论 砂生槐水提取物对免疫抑制型小鼠B淋巴细胞功能和巨噬细胞吞噬功能有增强作用。 【关键词】 砂生槐 水提取物 免疫抑制 B淋巴细胞 巨噬细胞 　　Abstract:ObjectiveTo explore the effect of Sophora moorcroftiana on the immune-function in the model of immunosuppressive mice. MethodsThe mouse model of inhibition was established induced by cyclophosphamide. After taken orally the aqueous extracts of sophora moorcroftiana〔0.6～2.4 g/(kg·d)〕, antagonistic experiments were observed in vivo, the spleen index and thymus gland index, proliferation index of B lymphocyte , the production of sheep red blood cell (SRBC) specific antibody ,phagocytosis of the phagocytes were used as evaluated indexes. ResultsThe low, middle, high doses of the aqueous extracts of Sophora Moorcroftiana could significantly improve immune function, compared with model groups ,Sophora moorcroftiana 〔0.6～2.4 g/(kg·d)〕 could obviously increase the spleen index; antagonize inhibition of immunological function caused by cyclophohphamide, promote B lymphocyte proliferation , the phagocytosis of the phagocytes. Sophora moorcroftiana 〔1.2～2.4g/(kg·d)〕 could also increase the production of sheep red blood cell (SRBC) specific antibody. ConclusionThe aqueous extracts of Sophora moorcroftiana seed has much promotion effect on immunity and phagocytosis of the phagocytes in suppressed mice. 　　Key words：Aqueous of Sophora moorcroftiana; Cyclophsphamide; Immunosuprpression; B lymphocyte; Phagocyte 　　砂生槐Sophora moorcroftiana又名“西藏狼牙刺”“刺柴”，豆科槐属多年生矮灌木，其茎尖和托叶硬化为尖长刺，为西藏高原一特有植物种。在雅鲁藏布江河谷呈大片分布，具有极强的抗旱、耐瘠薄、抗风沙等生态适应性和很好的防风固沙、保持水土功能［1］。藏医药记载［2］，砂生槐多以种子入药，味苦性凉，消炎解毒、催吐，主治湿热黄疸、白喉、痢疾、赤巴病、消化不良、虫病等，是一种极为宝贵的植物药用资源。本实验首次研究砂生槐水提取物对免疫抑制型小鼠B淋