碱性成纤维细胞生长因子对不同日龄大鼠癫疒间持续状态后海马神经发生的影响_医学论文.docVIP

碱性成纤维细胞生长因子对不同日龄大鼠癫疒间持续状态后海马神经发生的影响_医学论文 碱性成纤维细胞生长因子对不同日龄大鼠癫疒间持续状态后海马神经发生的影响_医学论文 作者：戴园园 袁宝强 魏海燕 【摘要】 目的 了解不同年龄大鼠癫疒间持续状态后外源性碱性成纤维细胞生长因子(bFGF)对海马齿状回颗粒细胞层神经发生的影响。方法 建立14天大鼠和60天大鼠戊四氮(PTZ)诱导癫疒间持续状态模型,生理盐水（NS）注射作为对照，皮下注射bFGF进行干预，分4组：NS组、NS+ bFGF组 、PTZ组、PTZ+ bFGF组。采用Brdu标记新生细胞，免疫组化方法检测造模后大鼠海马齿状回神经发生情况，造模后第7、14天2个时间点观察海马齿状回Brdu阳性细胞数。结果 造模后第7天和14天,PTZ组中各日龄组大鼠海马齿状回Brdu阳性细胞数明显高于各自对应的NS组(0.01),NS+bFGF组各日龄大鼠Brdu阳性细胞数亦高于NS组(0.05), PTZ+bFGF组Brdu免疫阳性细胞数较PTZ组亦有升高(14天日龄组0.05,60天日龄组0.01)。60天大鼠较14天大鼠升高幅度更为明显(0.01)。结论 bFGF可增加癫疒间持续状态大鼠海马齿状回颗粒细胞发生,对60日龄大鼠比14日龄幼鼠更为明显。 【关键词】 癫疒间持续状态 碱性成纤维细胞生长因子 神经发生 齿状回 Abstract: Objective To study the neurogenesis of hippocampal dentate gyrus granular cell layer in different-age rats with induced status epilepticus and its response to ectogenic basic fibroblast growth factor (bFGF). Methods Pentetrazole (PTZ) was used to form status epilepticus model on 14-day and 60-day old rats. The experiment was carried out in four groups, with the results analysed 7 days and 14 days after treatment. Brdu labeled new cells were counted to describe the neurogenesis in dentate gyrus. Results 7 days and 14 days after treatment, Brdu positive cells in the dentate gyrus were more numerous in the PTZ group and the NS+bFGF group than in the NS group, more numerous in the PTZ+bFGF group than in the PTZ group (0.01, 0.05）. The increment was more marked in the 60-day-old rats than in the 14-day-olds. Conclusion Basic fibroblast growth factor can promote neurogenesis in the hippocampus dentate gyrus granular cell layer in rats with status epilepticus, and the change is more marked in the elder rats than in the younger. Key words: status epilepticus basic fibroblast growth factor neurogenesis dentate gyrus 癫疒间是神经系统常见疾患，发作后海马齿状回可发生多种可塑性变化，包括轴索重组、星形胶质细胞活化、树突重组和颗粒细胞的神经发生(neurogenesis)等。神经发生是指包括人在内的大多数哺乳类动物均存在的神经系统产生新神经元的过程。近年来，已有研究证实癫疒间发作后海马齿状回颗粒细胞层的神经发生增加［1－2］。海马齿状回颗粒细胞层的神经发生受到多种生长因子和神经营养因子的调控，其中碱性成纤维细胞生长因子(basic fibroblast growth factor, bFGF)是较重要的一种。本试验即给予外源性bFGF皮下注射，探讨bF