白腐真菌培养条件对其分泌木质素降解酶的影响.pdfVIP

中国环境科学 2005,25(5)：572~575 China Environmental Science 白腐真菌培养条件对其分泌木质素降解酶的影响 * 高大文,文湘华,钱 易 (清华大学环境科学与工程系,环境模拟与污染控制国家重点实验室,北京 100084) 摘要：应用摇瓶试验研究了不同白腐真菌培养条件对其产酶的影响.通过控制不同转速、投加载体等方式考察了白腐真菌的产酶情况.结 果表明,在悬浮培养方式下,转速为 160r/min 时获得的锰过氧化物酶最高(481.6U/L),其酶活是静置、120r/min 条件下培养的65 倍和43 倍; 投加载体后,载体固定化培养获得的木质素过氧化物酶酶活是悬浮培养的 71 倍,载体投加量对其产酶影响很大,只有当载体处于非浸没状 态时才有利于酶活产生,否则酶活极低.因此,选择载体固定化—非浸没—振荡培养方式培养白腐真菌可获得更高的产酶量和酶活. 关键词：白腐真菌；形态；固定化；木质素过氧化物酶(LiP)；锰过氧化物酶(MnP) 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2005)05-0572-04 * Influence of culturing conditions of white rot fungus on the secretion of ligninolytic enzymes. GAO Da-wen , WEN Xiang-hua, QIAN Yi (State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, China). China Environmental Science, 2005,25(5)：572~575 Abstract：The influence of different culturing conditions of white rot fungus P.chrysosporium on the production of enzymes was studied appling the experiment of shaking flasks. The condition of enzyme production of white rot fungus was inspected through controlling different rotation speed, throw carriers type. In the type of suspended culture, manganese peroxidase attained was the highest (481.6U/L) when the rotation speed was 160r/min . Its enzyme activity was 65 times and 43 times of that cultured at the rotation speed of 0 and 120r/min. After throw addition of carrier, the activity of ligninolytic peroxidase obtained in carrier immobilized culture was 71 times of that in suspended culture; the throw addition amounts of carriers influenced greatly the production of enzymes. The carriers were favorable to the production of enzyme activity