小麦抗条锈病分子生物学和转基因育种研究进展.pdfVIP

西 南 农 业 学 报 2002 15 4 96 Sout hw est China Journal of A gricult ural Sciences V ol15 N o4 : 1001- 4829 ( 2002) 04 - 0096- 05 1, 2 1, 2 1, * 李跃建 , 朱华忠 , 刘世贵 ( 1, 6 1004 1; 2 , 6 10066) : 小麦条锈病是直接影响小麦高产稳产的关键性病害之 一, 也是世界性病害已发现30 个Yr 抗病基因, 且 位在相应染 色体上有些 Yr 基因的表达受环境影响; 抗性基因的不同组合影响抗性表现迄今已有20 多 个植物的抗病基因被克隆出来, 但 克隆的抗条锈病基因很少钙离子通道和激酶等在抗病基因的信号传导中起作用抗性基因多带有N BS 和L RR 等结构复制 重组转座子在调节区和编码区插入或切割等都可使抗性基因进化用RFLP 已找到 Yr 15 和 Yr 182 个抗病基因标记, 且进行了 位用R AP D 发现 Yr 8Yr 15 和YH52 标记, 用AFLP 找到 Yr 5Yr 10 和 YrH52 连锁标记目前, 只有 Yr 10 的抗性蛋白被发 现, 叫 亮氨酸拉链! 核苷酸连接位点富含亮氨酸蛋白 : 小麦抗条锈病; 基因; 表达; 分子标记; 抗性蛋白 : S5 12 : A Research advance of molecular biology and transgene breeding of wheat resistance to Puccinia striif ormis LI Yueian 1, 2 , ZHU Huazhong 1, 2 , LIU S hi gui1, * ( 1Life Science College, Sichuan U niversity , Chengdu 6 10041, China ; 2Sichuan Academy of Agricult ural S ciences, Chengdu 6 10066, China) Abstract : W heat st ripe rust was one of worldw ide key diseases, w hich af fected high and st able y ields of w heat. T he st udies showed 30 re sist ant gene Yr w ere found and locat ed on the corresponding chromosom es. U p t o now , resist ant genes f rom more t han 20 plants w ere cloned, but clones of resist ant gene to st ripe rust were few . Ca2+ pat hw ay s and K inase play ed roles in signal conduction of resist ant gen e. M ost of resistant gene had t he struct ures of N BS and L R R, and resistant gene could evolve by t he insertion and cleavage of replicat ion, re combinat ion and t ransposon in regulatory region and coding region. 2 resist ant