小麦显性多子房基因的RAPD标记研究.pdfVIP

业生物技术学报 Journal of Agricultural Biotechnology 2005,13 (5): 553~556 ·研究论文· 小麦显性多子房基因的RAPD 标记* 军卫 张改生** 刘宏伟 宋亚珍 牛 娜 ， (西北农林科技大学陕西省作物杂种优势研究与利用重点实验室 杨陵712100) 邾 ，从 摘要: 利用BSA 法，以显性多子房小麦( )与普通小麦( ) 种西 1376 进行杂交 F2 分离世 代建立多子房性状基因池和单子房基因池，采用RAPD-PCR 方法，以440 个随机引物筛选单、多子房的混合群体及其双亲。发 现10 个引物的扩增产物在近等基因系间表现出特异性, 表现为多子房亲本及其混合群体有带，单子房亲本及其混合群体无 带；6 个引物扩增产物表现出的特异性相反，单子房有带，多子房无带。经过重复实验和群体单株验证，引物S236 在多子房亲 混 ，可以作为多子房显性基因的分子标记。 本、 合群体及其群体单株中都表现出稳定的多态性 邾 关键词: 小麦; 显性; 多子房基因; RAPD Detection of A RAPD Marker Linked to Dominant Multi-ovary Gene in Wheat ( ) WANG Jun-Wei ZHANG Gai-Sheng** LIU Hong-Wei SONG Ya-Zhen Niu Na 邾 Using BSA method, a dominant multi-ovary line of common wheat ( ) was crossed with variety Xi- nong 1376 ( ). From F2 segregation population, a multi-ovary and a single-ovary gene pools were established; via RAPD-PCR method, single-ovary and multi-ovary mixed populations were screened using 440 random primers. The result indicated that the expanded product of 10 primers showed specific character among neaisogenic lines, multi-ovary population and its parent had band, while single-ovary population and its parent didnt. The expanded product of 6 primers showed the opposite character that the product of single-ovary line