不同提取工艺对家蝇幼虫蛋白粗提液抗菌活性的影响.pdfVIP

( ) 昆 虫 学 报 Acta Entomologica Sinica , October 2004 , 47 5 : 691 - 694 ISSN 不同提取工艺对家蝇幼虫蛋白粗提液抗菌活性的影响 安春菊 , 耿　华 , 郝友进 , 李德森 , 杜荣骞 (南开大学生命科学学院 , 天津　300071) 摘要 : 利用不同条件提取诱导过的家蝇 Musca domestica 3 龄幼虫的总蛋白 ,并用平板扩散法测定所得蛋白粗提液的抗菌活性。 结果表明 ,收集幼虫时的处死温度、沸水浴时间及提取液的pH 值均对家蝇幼虫粗提液的抗菌活性有明显的影响。 关键词 : 家蝇 ; 蛋白粗提液 ; 提取工艺 ; 抗菌活性 ( ) 中图分类号: Q966 　　文献标识码 : A 　　文章编号 : 2004 The influences of different factors in extraction on antibacterial activity of protein crude extracts in housefly larvae AN Chun-Ju , GENG Hua , HAO You-Jin , LI De-Sen , DU Rong-Qian ( College of Life Sciences , Nankai University , Tianjin 300071 , China) Abstract : The influences of different factors in extraction on the antibacterial activity of protein crude protein extracts from the induced third-instar larvae of housefly ( Musca domestica ) were assayed. The antibacterial activity of protein crude extracts was measured with diffusion plate assay. The results showed that the antibacterial activity of crude extracts was affected by such factors as the temperature of boiling larvae to death , the duration of boiled water bath and the pH of extraction buffer. Key words : Housefly larvae ( Musca domestica) ; protein crude extracts ; extraction protocol ; antibacterial activity 　　家蝇 Musca domestica 是一种重要的资源昆虫 , 13 　幼虫的处死温度 具有来源丰富、饲养方便和繁殖力强等优点 ,王远程 将样品分别于 80 ℃、85 ℃、90 ℃、95 ℃和 100 ℃水 ( ) ( ) 等 1997 和陈留存等 2001 曾从家蝇幼虫中分离到 浴中加热 5 min 后收集 , - 20 ℃保存准备提取蛋白 , 与抗菌肽类似的物质。如果找到一种高效的提取工 提取缓冲