Comparative Genomic Hybridization(CGH).pptVIP

Comparative Genomic Hybridization(CGH) Outline Introduction to gene copy numbers and CGH technology DNA copy number alterations in breast cancer (Pollack et.al., PNAS (2002)) Copy number polymorphism in human genomes (Sebat et.al., Science (2004)) Alteration in DNA Copy Number: amplification and deletion Alteration in DNA Copy Number: possible mechanism Array Based Comparative Genomic Hybridization Goal: to detect copy number alterations using a gene chip Ideally, the signal intensity is proportional to copy number Several genomes can be compared simultaneously Technical consideration in