基于纳米银催化放大效应分子印迹传感器研究的论文.pdfVIP

A034 基于纳米银催化放大效应分子印迹传感器研究 尹伟玲，李建平* 桂林理工大学 化学与生物工程学院，桂林 541004 摘 要：基于纳米银粒子催化金属沉积放大效应，结合分子印迹技术和阳极溶出伏安法，建立了灵敏测定吲哚乙酸的方 法。以吡咯为功能单体，采用电聚合方法在玻碳电极表面，制备吲哚乙酸分子印迹聚合物膜，利用吲哚乙酸与纳米银粒 子标记物的竞争反应和纳米银催化铜沉积实现对吲哚乙酸的检测。吲哚乙酸浓度在9×10-10 ~ 6×10-7 mol/L 范围内时，溶 出峰电流与浓度对数呈良好的线性关系，检出限（S/N = 3）为2.3×10-10 mol/L 。对样品中吲哚乙酸进行检测，回收率在 99.7 % ~ 104.2 %之间。 关键词：分子印迹；纳米银粒子；吲哚乙酸；阳极溶出伏安法；催化沉积 Molecularly imprinted sensor based on the catalytic amplification by nanosilver , YIN Wei-ling , LI Jian-ping* College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China Abstract: A novel strategy for the sensitive determination of 3-indoleacetic acid (IAA) was proposed based on molecular imprinting and amplification of nanosilver-catalyzed deposition with linear sweeping voltammetry (LSV). An polypyrrole (PPY) film was electrochemically polymerized on a glassy-carbon electrode surface to fabricate an MIP electrochemical sensor using IAA as a template, the determination of IAA based on the competitive reaction and the catalytic reduction of copper deposition by nanosilver. The anodic stripping peak current was proportional to the logarithm of the concentration of IAA in the range of 9.0×10-10 ~ 6.0×10-7 mol/L with a detection limit of 2.3×10-10 mol/L attained by 3σ-rule. The recovery rate of the approach is in the range of 99.7 % ~ 104.2 %. Keywords: molecular imprinting; nanosilver; 3-indoleacetic acid; linear sweeping voltammetry; catalyzed deposition 植物生长调节激素吲哚乙酸(IAA)，在植物体内含量甚微，却对植物生长发育有重要影响，直接参 与植物的向性、顶端优势、须根分裂等生命活动。吲哚乙酸在农业上的应用日渐增多，掌握其在植物体 [1] 内的变化规律，对研究植物生理过程和植物培育至关重要。传统液相色谱、离子色谱荧光等方法 测定 吲哚乙酸，耗时长，容易造成样品失真，引起较大误差。分子印迹电