多株功能菌的混合培养.pdfVIP

2009 15 4 ): 523~528 2009-08-25 应用与环境生物学报 ， ( Chin J Appl Environ Biol=ISSN 1006-687X DOI: 10.3724/SP.J.1145.2009.00523 多株功能菌的混合培养* ** 冯 栩 李旭东 曾抗美 刘庆华 (中国科学院成都生物研究所 成都 610041) 摘 要 以自行研制的功能菌为对象，通过菌体生长曲线与基质消耗曲线的比较与分析，研究不同菌混合培养时各菌 的生长与单一培养时的差异. 结果显示，功能菌 FPN、FEm、FCB和F10单一培养时，菌体生长过程服从修定的Logistic 方程，最大比生长速率分别为0.248 h-1 -1 -1 -1 、0.207 h 、0.196 h 和0.348 h ；当FPN和FEm为一组、F10和FCB为一组进行两株 菌混合培养时，由分离培养基计数得到的各种菌的生长曲线与对应的该菌单一培养时的生长曲线无显著差异，各组 的基质消耗曲线与各自对应的菌单一培养时的消耗曲线之和无显著差异；当FPN、FEm、FCB、F10这4株菌混合培养 时，混合菌体的生长曲线与单一培养时的菌体生长曲线之和无显著差异，碳源消耗曲线与各菌单一培养时的碳源消耗 曲线之和无显著差异. 图6 表3 参21 关键词 功能菌；菌剂；混合培养；抑菌试验；动力学 CLC X172 : X703.5 Process of Mixed Culture of Functional Strains* FENG Xu, LI Xudong**, ZENG Kangmei LIU Qinghua (Chengdu Institute of Biology ,Chinese Academy of Sciences, Chengdu 6l004l, China) Abstract The differences in growth of functional strains that were cultivated in mixed culture and monoculture were compared by analysis of their growth and consumption curves. The results showed that when strains FPN, FEm, FCB and F10 were in monoculture, their processes of growth fi tted the modifi ed logistic equation, and the calculated maximum specifi c growth rates were 0.248 h-1 -1 -1 -1 , 0.207 h , 0.196 h and 0.348 h , respectively. When two stains were in mixed culture in pair of FPN and FEm, and that of F10 and FCB, each strain could be counted by using selective media, and the corresponding growth