子宫内膜癌I型和II型全基因表达谱初步分析_百替生物.pdfVIP

子宫内膜癌I 型和II 型全基因表达谱初步分析 1 1 1 1 1 作者：郭遂群；邢福祺；庞占军；方唯意；刘国炳 (南方医 1 2 科大学南方医院妇产科，肿瘤研究所，广东 广州 510515) 摘要：目的 分析子宫内膜癌I 型和II 型全基因表达谱。方法 利用6 张Affymetrix 全基因组基因芯片，分析两者之间在表达谱上的差异，结合生物信息分析。结果 许 多基因明显在雌激素依赖型子宫内膜癌中高表达，其中，一些基因涉及了雌激素依赖 型中雌激素的代谢与转化，另外一些基因可能为雌激素调节基因。CYP2C9 参与了雌 激素酮硫酸盐转化为16-羟基硫酸盐代谢；DDC 能与类固醇受体结合，增强类固醇受 体的转录活性。结论 某些基因在雌激素依赖型子宫内膜癌中高表达，为进一步研究 此类基因在子宫内膜癌发病过程的作用及预后奠定了基础。 关键词：子宫内膜肿瘤；基因芯片；生物信息分析；雌激素依赖型 中图分类号：R737.33; R392.3 文献标识码：A 文章编号： 1673-4254(2006)06-0734-04 Preliminary study of gene expression profiling in human type I and II endometrial carcinoma GUO Sui-qun1 1 2 1 ； XING Fu-qi ； PANG Zhan-jun1； FANG Wei-yi ； LIU Guo-bing 1 2 Department of Gynaecology and Obstetrics, Nanfang Hospital, Institute of Cancer Research, Southern Medical University, Guangzhou 510515, China Abstract: Objective To study gene expression profiling in human type I and II endometrial carcinoma. Methods Six Affymetrix human genome genechips were utilized to investigate the differences in gene expression profiles between type I and II endometrial carcinoma with bioinformatic analysis. Results Many genes were highly expressed in estrogen-dependent endometrial carcinoma, and service@100 some of them were involved in the metabolism and conversion of estrogen, while some others in estrogen regulation. CYP2C9, for instance, was involved in the conversion of estrogen sulfate to 16-hydroxy sulfate metabolite, DDC in estrogen-dependent pathogenesis of endometrial carcinom