加替沙星和环丙沙星体外抗生素后效应期间大肠埃希菌菌体大小及DNA含量变化研究.pdfVIP

加替沙星和环丙沙星体外抗生素后效应期间 大肠埃希菌菌体大小及DNA含量变化研究 朱曼 王睿 张永青 梁蓓蓓 中国人民解放军总医院临床药理药学研究室 北京 100853 摘要 目的 研究加替沙星和环丙沙星体外抗生素后效应期间E.coli ATCC25922 菌体大小及DNA 含量变化 为探讨 PAE 形成机理提供实验依据 方法 于加替沙 星及环丙沙星体外PAE 期间不同时间点吸取培养物 以荧光探针碘化丙啶进行染色 经流式细胞仪分析E.coli ATCC25922 菌体大小及DNA 含量变化 结果 加替沙星和 环丙沙星PAE 期间E.coli ATCC25922 菌体显著增大 胞内DNA 含量明显增加 且 呈剂量依赖性 当加替沙星与蛋白合成抑制剂氯霉素及RNA 合成抑制剂利福平联用 时 这一变化被抑制 结论 应用流式细胞仪辅以荧光探针碘化丙啶可检测加替沙 星及环丙沙星PAE 期间细菌菌体大小和DNA 含量变化 该方法快捷 简便 直观 关键词 加替沙星 环丙沙星 抗生素后效应 大肠埃希菌 The change of sizes and nucleic acid contents of Escherichia coli during the Postantibiotic effect after exposure to gatifloxacin and ciprofloxacin Zhu man, Wang rui, Zhang yong-qing, Liang bei-bei (Department of clinical pharmacology, Chinese PLA General Hospital Beijing 100853 ) ABSTACT: OBJECTIVE We study the change of sizes and nucleic acid contents of Escherichia coli during the Postantibiotic effect after exposure to gatifloxacin and ciprofloxacin in order to investigate the mechanism of PAE. METHODS During the Postantibiotic effect after exposure to gatifloxacin and ciprofloxacin, the aliquots were taken from the bacterial culture at regular interverals. Then We employed flow cytometry in conjunction with fluorescent probes to follow the dynamic change of sizes and nucleic acid contents of Escherichia coli. Results The sizes of Escherichia coli were different from those of the control population. In parallel, an increase in nucleic acid contents was still noted at the end of tne experiment. This change was inhibited by the protein synthesis inhibitor Chloramphenicol and the RNA synthesis inhibitor Rifampicin. CONCLUSION: Gatifloxacin and Ciprofloxacin induced filamentation and the increase of nucleic acid contents of Escherichia coli were inhibited by the protein synthesis