激发子诱导下合作杨细胞钙_氢离子流的振荡.pdfVIP

2011, 20 9 : 155- 159 A cta A g r ic ult ur ae B or eali-occ idental is S in ica * 激发子诱导下合作杨细胞钙氢离子流的振荡 1, 2 1 高海波 , 沈应柏 1. , 100083; 2. , 276002 + : 2 , H 2+ + 2+ Ca , 2 H Ca , + 2+ + 2+ 2 H , Ca ; 2 H Ca : ; ; ; : Q257 : A : 1004- 1389 2011 09-0155-05 Calcium and Proton Flux Oscillations Induced by Elicitors in P. simonii P. p y ramidalis Opera 8277 Cells GA O H aibo1, 2 and HEN Yingbai1 1. College of Biological ciences and Biotechnology, Beijing Forestry U nivershity, Beijing 100083, China; 2. College of Life cience, Linyi University, Linyi handong 276002, China Abstract: P . s imoni i P. p y r ami dalis Opera 8277 cells w ere treated w ith elicitor of M e A and + 2+ MeJA, and net ion fluxes of H and Ca w as measured by the method of Non-invasive micro-test + 2+ technique. The result show ed that tw o kinds of elicitor induced oscillations of H flux and Ca flux in the w hole-cell level, the integrated ion flux suggested that tw o kinds of elicitor induced H+ net ef- fulx and Ca2+ net influx , autocorrelation function of ion flux show ed that the period of oscillations w ere similar to each other under the treatment of tw o kind of elicitors. Key words: P. simonii P. p yr amidal is Opera 8277; Non-invasive micro-test technique; Ion flux; Elicitor [ 5] , , [ 6] Me A [ 7] , , [ 1] , [ 8] M eJA M e A [ 2] 2 , [ 9] MeJA [ 3] , , [ 10] , [ 4] M eJA , * : 2010- 12-24 : 2011-04-05 : 座机电话号码 : , , , E-mail: gaohaib@ 126. com : , , , E-mail: ybshen@ bjfu. edu. cn 156 20 , , 2 , 60 [ 11] , M e- 1 h: , 2 + + A MeJA 2 , Ca H , 20 s , 3 , , 3 mL 1 h MatTek 1 材料与方法 , 1. 1 , P . s i moni i P. p y r ami dalis Opera 2~ 5 m: 8277 M , 10 m, 0. 2 Hz - 1 , 6-BA 1 mg L 5 min , 3 mL NAA 1 mg L- 1 30 g L- 1 pH 5. 8 Me A MeJA , - 1 , 160 r min 25 M e A M eJA 100 7 d mol L- 1 , 50 min; CK , , 3 mL 7 d 1 mL 1. 50 mL M , Mageflux ht- tp:/ / xuyue. net/ mageflux , , 1. 2 , 2+ + Ca H , 2 min , BIO-IM NMT , 0. 2 Hz, 6 0.