虫草素对肿瘤坏死因子α诱导的大鼠血管平滑肌细胞增殖及syndecan4蛋白表达的影响_药学论文.docVIP

虫草素对肿瘤坏死因子诱导的大鼠血管平滑肌细胞增殖及syndecan蛋白表达的影响_药学论文 虫草素对肿瘤坏死因子诱导的大鼠血管平滑肌细胞增殖及syndecan蛋白表达的影响_药学论文 作者：张宇，欧阳平，赖文岩，贝伟剑2，许顶立 【摘要】 目的 观察虫草素对肿瘤坏死因子刺激的离体大鼠血管平滑肌细胞(VSMCs)增殖和syndecan蛋白表达的影响。 方法 体外培养大鼠胸主动脉VSMCs，分别应用终浓度为20 ng/mL TNF、20 μmol/mL虫草素、40 μmol/mL虫草素、20 ng/mL TNF虫草素、20 ng/mL TNF虫草素作用24 h，并设对照组进行比较。采用MTS/PMS法确定VSMCs的增殖状态，利用Western blot蛋白免疫印迹法测定VSMCs中syndecan蛋白的表达。结果 (1)与对照组比较，TNF组能显著刺激大鼠VSMCs的增殖(0.05)，而虫草素不同剂量组单独应用对大鼠VSMCs的增殖均无明显作用(Pgt;0.05)。TNF联合虫草素共同作用组与TNF组比较， VSMCs增殖均显著减少(0.05)。(2)Western blot蛋白免疫印迹法测定显示：与对照组比较，TNF组能显著刺激VSMCs的syndecan蛋白表达(0.05)，而虫草素不同剂量组单独应用对VSMCs的syndecan蛋白表达均无明显影响(Pgt;0.05)。TNF联合虫草素共同作用组与TNF组比较，VSMCs的syndecan蛋白表达显著降低(0.05)。结论 虫草素可明显抑制TNF诱导的VSMCs 增殖及细胞syndecan蛋白的表达。 【关键词】 syndecan虫草素;肿瘤坏死因子α;血管平滑肌细胞;细胞增殖 Abstract:Objective To investigate the effects of cordycepin on the proliferation of rat vascular smooth muscle cells(VSMCs)and expression of syndecan，20 μmol/mL cordycepin，40 μmol/mL cordycepin，20 μmol/mL cordycepin with 20 ng/mL TNF，and 40 μmol/mL cordycepin with 20 ng/mL TNF，respectively. All groups were treated for 24 h in vitro,in addition to the control group established for comparison. The ratio of proliferation of VSMCs was determined by nonthe proliferation of rat VSMCs at the concentration of 20 ng/mL in TNFα group (0.05). Cordycepin alone had no effect on VSMCs growth (Pgt;0.05),but significantly inhibited VSMCs proliferation induced by TNFα (0.05). (2) Compared to the control group,the expression of syndecan4 protein in VSMCs was significantly enhanced by TNFα at the concentration of 20 ng/ml (0.01). Cordycepin alone had no effect on the expression of syndecan4 protein (Pgt;0.05),but significantly inhibited the expression induced by TNFα (0.01).Conclusion Cordycepin could inhibit cell proliferation and expression of syndean4 protein in rat VSMCs induced by TNFα in vitro. 　　Key words:syndecan 　　血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖和表型改变是动脉粥样硬化(atherosclerosis,AS)的主要病理基础之一，动脉粥样硬化是对血管损伤的过度炎症反应。肿瘤坏死因子，TNF是炎症反应的关键调节因子之一，可从多种不同的炎症